Comparison of the Biofire FilmArray Respiratory Panel, Seegene AnyplexII RV16, and Argene for the detection of respiratory viruses

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Maurice ChanThean Yen Tan

Abstract

Respiratory infections are common reasons for hospital admission, and are associated with enormous economic burden due to significant morbidity and mortality. The wide spectrum of microbial agents underlying the pathology renders the diagnosis of respiratory infections challenging. Molecular diagnostics offer an advantage to the current serological and culture-based methods in terms of sensitivity, coverage, hands-on time, and time to results. This study aimed to compare the clinical performance of three commercial kits for respiratory viral detection. The performance of FilmArray Respiratory Panel, AnyplexII RV16, and Argene was compared using clinical respiratory samples (n = 224, comprising 189 nasopharyngeal swabs in Universal Transport Medium (UTM) and 35 endotracheal aspirates), based on common overlapping targets across the platforms. Influenza A "equivocal" and "no-subtype" samples by FilmArray were further compared to a laboratory-developed Influenza A/B test. The overall performance of all three platforms appeared to be comparable with regards to sensitivities (95.8-97.9%) and specificities (96.1-98.0%), detection of coinfections, and distinguishment of influenza from non-influenza cases. "Equivocal" and "no-subtype" ...Continue Reading

Citations

Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Susanna EspositoNicola Principi
Oct 16, 2020·Nature Reviews. Microbiology·Olivier VandenbergZisis Kozlakidis
Jan 20, 2021·Revue des maladies respiratoires·S PilletA Vabret
Dec 30, 2020·Microorganisms·Sonia BianchiniSusanna Esposito
Feb 28, 2021·Diagnostic Microbiology and Infectious Disease·Justin HardickUNKNOWN Emergency Department National Network Influenza Investigators
Feb 21, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Charlotte PronierVincent Thibault
Mar 23, 2021·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·Ivan SanzJosé María Eiros
Oct 10, 2021·Scientific Reports·Cong-Tat CiaWen-Chien Ko

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Datasets Mentioned

BETA
AB7500

Methods Mentioned

BETA
PCR
nucleic acid amplification
bronchoalveolar lavage

Software Mentioned

FA
eSensor
Biofire
Filmarray
AnyplexII
xTAG
RP
Argene
RespiFinder

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