Comparison of the bioMérieux NucliSENS EasyQ HIV-1 v2.0-HIV-1 RNA quantification assay versus Abbott RealTime HIV-1 and Roche Cobas TaqMan HIV-1 v2.0 on current epidemic HIV-1 variants

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Thomas MourezJean-Christophe Plantier

Abstract

An improved version of the bioMérieux NucliSENS(®) EasyQ(®) HIV-1 v2.0 has been introduced to overcome the underquantification observed with previous versions, especially with non-B HIV-1 subtypes. Comparing bioMérieux NucliSENS(®) EasyQ(®) HIV-1 v2.0 versus Roche Cobas CA/CTM v2.0 and Abbott RealTime HIV-1 assays for HIV-1 group M and non-M (N, O, P) viral load measurement. The three assays were tested in parallel on 103HIV-1 group M plasma samples, and on non-group M HIV-1 culture supernatants. Values obtained for the 103HIV-1 group M plasma samples tested with bioMérieux assay showed good overall correlation compared to the 2 others. The Roche Cobas assay gave higher values than the bioMérieux assay, while Abbott and bioMérieux both displayed similar results. However, analysis showed a wider dispersion in results when comparing the bioMérieux NucliSENS(®) EasyQ(®) HIV-1 v2.0 and the other 2 techniques. All data taken into account, we observed frequent discrepancies in quantification, of the plasma samples and major differences above 1log in 10/72 (13.8%). The quantification of non-M HIV groups in culture supernatant has shown variable results, with better quantification of HIV-O and of HIV-N respectively with the Abbott assa...Continue Reading

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Citations

Dec 14, 2017·AIDS Research and Human Retroviruses·Mary A RodgersGavin Cloherty
Mar 17, 2018·AIDS·Elodie Alessandri-GradtJean-Christophe Plantier
Dec 22, 2016·The Journal of Antimicrobial Chemotherapy·Marc WirdenAnne-Genevieve Marcelin
Feb 25, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Junpeng ZhaoLunan Wang
Aug 16, 2017·Analytical and Bioanalytical Chemistry·Payam HashemiBettina Wünsch
Jun 21, 2017·PeerJ·Dean R VikMatthew B Sullivan
Mar 24, 2016·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Marie GueudinJean-Christophe Plantier
Sep 11, 2021·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Elodie Alessandri-GradtUNKNOWN RES-O Network
Mar 19, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A BergerO T Keppler

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