Comparison of the conformational state and in vitro refolding of yeast chaperonin protein cpn10 with bacterial GroES

Biochemical and Biophysical Research Communications
H H de JonghC M Dobson

Abstract

Gel filtration studies demonstrate that the heptameric complex of yeast cpn10 (pI around 8.8) reversibly disassembles into monomers when lowering the pH to 4.5, whereas its secondary structure is retained as demonstrated by circular dichroism. Monomeric yeast cpn10 does not bind to GroEL in the presence of nucleotides, whereas under identical conditions E. coli cpn10 (GroES), having a strong sequence homology to the yeast form but a pI of 5.2, shows no pH-dependent dissociation and is able to complex with GroEL at both pH 7.5 and 4.5. Using circular dichroism it is shown that, unlike E. coli cpn10, yeast cpn10 is not able to refold spontaneously after first being fully unfolded in 8 M urea. However, refolding of yeast cpn10 to a complex that can be recognised by GroEL depends on the presence of a lipid-water interface with a specificity for negatively charged lipids. We suggest that the requirements for refolding of yeast cpn10 are related to its post-translational transport and subcellular localization.

References

Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K TillyC Georgopoulos
Aug 22, 1995·Biochemistry·J ZondloE Eisenstein
Sep 22, 1995·The Journal of Biological Chemistry·J Ybarra, P M Horowitz
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S RospertP V Viitanen
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·F J Corrales, A R Fersht
Apr 2, 1996·Biochemistry·J W SealeP M Horowitz
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y DubaquiéS Rospert
Nov 1, 1978·Analytical Biochemistry·C T ChangJ T Yang

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