Comparison of the cytotoxic effects of cadmium (Cd(2+)) in high and low resistance strains of MDCK cells that express different levels of E-Cadherin

Toxicology in Vitro : an International Journal Published in Association with BIBRA
W C Prozialeck, P C Lamar

Abstract

Previous studies from our laboratory have shown that cadmium (Cd(2+)) can disrupt the adhering and occluding junctions between MDCK cells. Recently, we have obtained evidence to suggest that Cd(2+) produces this effect by interacting with E-cadherin, a Ca(2+)-dependent cell adhesion molecule that is localized at the adhering junctions of epithelial cells. The objective of the present study was to examine the junctional and cytotoxic effects of Cd(2+) in subcloned strains of MDCK cells that express different levels of E-cadherin. One strain (MDCK I) expresses high levels of E-cadherin and develops a transepithelial electrical resistance of more than 800 Omega.cm(2), whereas the other strain (MDCK II) expresses much lower levels of E-cadherin and develops a transepithelial resistance of less than 100 Omega.cm(2). The results showed that exposure to 20 mum Cd(2+) for 2-4 hours caused a pronounced loss of E-cadherin from the cell borders in both strains of cells. In the MDCK I cells, the loss of E-cadherin coincided with a decrease in the transepithelial electrical resistance and the loss of the tight junction-associated proteins, ZO-1 and occludin, from the cell borders. By contrast, the MDCK II cells first exhibited a significant...Continue Reading

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Citations

Feb 7, 2003·Life Sciences·Celeste A PearsonWalter C Prozialeck
Jun 7, 2003·Toxicology and Applied Pharmacology·Walter C ProzialeckSean M Lynch
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M F AleoG Mazzoleni
Jun 13, 2006·Life Sciences·Walter C ProzialeckJames M Woods
May 9, 2000·Toxicology and Applied Pharmacology·W C Prozialeck

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