Comparison of the cytotoxic impact of chlorfluazuron on selected insect and human cell lines

Environmental Toxicology and Chemistry
Qingchun HuangLiming Tao

Abstract

To gain new insight into the mechanism of selective cytotoxicity of benzoylureas as insecticides, the in vitro mode of action of chlorfluazuron was investigated on lepidopteran Tn5B1-4 and Sf-21 cells and human Hek293 and HepG2 cells. Chlorfluazuron inhibited the proliferation of Tn5B1-4 and Sf-21 cells with 50% inhibitory concentration values (IC50) of 4.96 µM and 1.12 µM at 48 h and 2.37 µM and 1.76 µM at 96 h, respectively, versus that of Hek293 and HepG2 cells with IC50 values >20 µM. When transferred to chlorfluazuron-free medium, lepidopteran Tn5B1-4 and Sf-21 cells had a postinhibitory recovery development period within 24 h followed by a suppressed increase in cell viability, but human Hek293 and HepG2 cells showed an accelerated increase over their control level. Chlorfluazuron affected Tn5B1-4 and Sf-21 cells, with ≥1.8-fold decreases in the ratio of cellular N-acetylglucosamine (GlcNAc) level and protein content and ≥1.5-fold increases in the mitotic index and G2 /M-phase arrest. Neither Hek293 nor HepG2 cells contained GlcNAc, and chlorfluazuron had no significant effects on the cell cycle and mitotic index of Hek293 and HepG2 cells. In conclusion, the differences between human and lepidopteran cell lines in the cha...Continue Reading

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Citations

Dec 13, 2016·Ecotoxicology and Environmental Safety·Xinming YunZiyi Wan
Apr 1, 2020·Journal of Biochemical and Molecular Toxicology·Rim TimoumiSalwa Abid-Essefi
Aug 21, 2020·Pest Management Science·Michelle MakRobert Spooner-Hart

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