Abstract
Most critical instruments are not designed for heat sterilization and autoclaving. These items are usually treated with chemical agents such as peracetic acid(PAA), glutaraldehyde (GA) and ortho-phthalaldehyde (OPA). MTT assay is often used to evaluate the in vitro cytotoxicity of these chemical agents. In this study, disinfectants were allowed to come in direct contact with cells. Their cytotoxicity was evaluated based on cell viability and adhesive properties. The results obtained from the direct contact method were compared with those obtained from the conventional MTT assay wherein the disinfectants were added into a nutrient medium. It was found that the two methods yielded very different results, especially when aldehyde- and halogen-containing disinfectants were tested, and that toxicity may be underestimated in the MTT assay. Hence, it can be assumed that the direct contact assay is more accurate when evaluating the cytotoxicity of residual chemicals. It was also observed that the cytotoxicity of PAA was lower than that of GA and OPA.
References
Apr 1, 1982·The Journal of Pharmacy and Pharmacology·H Y Cheung, M R Brown
Apr 1, 1983·The Journal of Applied Bacteriology·P Gélinas, J Goulet
Apr 1, 1995·Gastroenterology·A B WestS Lagarde
Sep 1, 1994·Gastrointestinal Endoscopy·P RozenT Gilat
Oct 13, 2001·Diseases of the Colon and Rectum·A Di CarloC A Vasilevsky
Oct 23, 2003·Anesthesia and Analgesia·Steven G VenticinqueRobert J O'Connell
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·William N Sokol
Feb 24, 2006·Dermatology : International Journal for Clinical and Investigative Dermatology·G Müller, A Kramer
Jun 6, 2006·The Journal of Allergy and Clinical Immunology·Maho SuzukawaKazuhiko Yamamoto
Dec 21, 2006·Journal of Occupational Health·Hiroshi FujitaYoko Endo
May 23, 2007·Archives of Dermatology·Farah R Abdulla, Brian B Adams
May 23, 2007·Regulatory Toxicology and Pharmacology : RTP·Masahiko AyakiAtsuo Iwasawa
Jun 22, 2007·Allergology International : Official Journal of the Japanese Society of Allergology·Maho SuzukawaMasao Yamaguchi
Aug 21, 2007·Journal of Endourology·Andrea CestariPatrizio Rigatti
Sep 25, 2008·Journal of Endourology·Daniel E CooperBrain K Auge
Oct 9, 2009·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·T HirschL Steinstraesser
Jul 14, 2010·Maternal & Child Nutrition·Setsuko TsutieTetsuya Inatome
Jan 13, 2012·American Journal of Industrial Medicine·V H ArrandaleD L Holness
Oct 25, 2012·Archives of Oral Biology·F G BassoC A de Souza Costa
Citations
Mar 30, 2016·The Journal of Surgical Research·Shuichi OginoShigehiko Suzuki
Jan 31, 2017·International Endodontic Journal·K S ViolaG Faria
Dec 3, 2014·Journal of Laboratory Automation·Bradley ClassWei Zheng
Jun 14, 2019·Annals of Plastic Surgery·Artur Ferronato SotoCristiane Machado Mengatto