Comparison of the effect of p65 siRNA and curcumin in promoting apoptosis in esophageal squamous cell carcinoma cells and in nude mice

Oncology Reports
Fang TianXiaoyan Zhang

Abstract

The activation of the NF-κB signaling pathway plays a critical role in carcinogenesis. The role of the NF-κB pathway in esophageal squamous cell carcinoma (ESCC) remains ill-defined. The objective was to detect whether p65siRNA and curcumin could promote ESCC cell apoptosis and increase the sensitivity of ESCC cells to chemotherapeutic drugs by inhibiting the NF-κB signaling pathway, and to compared these two treatments. In the present study, the status of the NF-κB pathway, in the two ESCC cell lines Eca109 and EC9706, was analyzed and the ability of p65 siRNA and curcumin alone or in combination with 5-FU to modulate this pathway in vitro and in vivo was investigated. The results showed that the NF-κB signaling pathway in the ESCC cell lines was constitutively activated. Both p65 siRNA and curcumin mediated suppression of activation of the NF-κB signaling pathway via inhibition of the expression of p65 or IκBα phosphorylation in ESCC cell lines. The cells treated with combination of p65 siRNA or curcumin and 5-FU revealed a lower cell viability and higher apoptosis compared to those treated with 5-FU alone. In a human ESCC xenograft model, p65 siRNA or curcumin and 5-FU alone reduced the tumor volume, respectively, but their ...Continue Reading

Citations

Sep 10, 2013·Surgical Oncology Clinics of North America·Derek J Erstad, James C Cusack
Jan 25, 2018·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Lai Nar TungNikki P Lee
Jan 10, 2019·Journal of Cellular Physiology·Keywan MortezaeeAmirhossein Sahebkar
Feb 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muthu K ShanmugamGautam Sethi
Oct 24, 2020·ACS Combinatorial Science·Milad AshrafizadehPooyan Makvandi
Nov 5, 2020·Recent Patents on Anti-cancer Drug Discovery·Saleh A AlmatroodiArshad Husain Rahmani

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