Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1

Veterinary Medicine International
Arunee ThanasarasakulpongNattawooti Sthitmatee

Abstract

Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH pu...Continue Reading

References

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Dec 5, 2003·Veterinary Microbiology·Entomack BorrathybayEiichi Kawamoto
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Mar 4, 2010·Veterinary Microbiology·Tamás HatfaludiBen Adler

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Citations

Apr 20, 2017·Avian Pathology : Journal of the W.V.P.A·Thanya VarinrakNattawooti Sthitmatee
Jun 18, 2020·Veterinary Medicine International·Anucha MuenthaisongNattawooti Sthitmatee
Nov 8, 2017·The Journal of Veterinary Medical Science·Korkiat MuangthaiNattawooti Sthitmatee

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Methods Mentioned

BETA
electrophoresis
affinity chromatography
protein assay
ELISA
size exclusion chromatography

Software Mentioned

SPSS
Stata SE

Related Concepts