PMID: 3308793Jan 1, 1987Paper

Comparison of the effects of a preliminary hepatic washing and of saponin on the intracellular penetration of peroxidase-labeled anti-rat albumin antibodies in hepatocytes

Histochemistry
A CazierG Feldmann

Abstract

The effect of a preliminary hepatic washing with saline before liver fixation either by perfusion or immersion was compared to the effect of saponin, a membrane-permeabilizing agent, in order to ascertain which procedure is best to obtain a homogeneous distribution of albumin-containing hepatocytes in the hepatic lobule. Albumin was located in the hepatocytes by peroxidase-labeled antibodies using light and electron microscopy. The efficacy of the two procedures on the intracellular penetration of labeled antibodies in liver sections was judged by preparing transverse ultrathin sections. Both procedures yielded similar results. Liver fixation by perfusion with saponin and without a preliminary washing, however, distributes albumin-containing hepatocytes more homogeneously in the hepatic lobule and enables labeled antibodies to penetrate more satisfactorily. In contrast, when the liver is fixed by immersion, the preliminary washing is the only way to obtain an even distribution of albumin-containing hepatocytes, as saponin is not effective under these conditions. In conclusion, the localization of albumin in the hepatocytes must be adapted according to the technique used to fix the liver.

References

Nov 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A GuillouzoJ F Chiu
Oct 1, 1975·The Journal of Cell Biology·G FeldmannJ P Benhamou
Jan 1, 1985·International Review of Cytology·G FeldmannE Rogier
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Oct 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F PignalG Feldmann
Jul 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S J BurwenS Dejbakhsh
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Yokota, H D Fahimi
Feb 1, 1964·The Journal of Cell Biology·Y HAMASHIMAA H COONS

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