PMID: 11910587Mar 26, 2002Paper

Comparison of the effects of continuous combined and sequential combined medroxyprogesterone acetate-estradiol treatment on the proliferation of MCF-7 cells

Climacteric : the Journal of the International Menopause Society
C LippertAlfred O Mueck

Abstract

The aim of the study was to examine the effects of two different, clinically relevant, hormone replacement therapy (HRT) regimen types, continuous combined and sequential combined, on breast cancer cells by means of an in vitro model. The study was carried out using the C21-progestin medroxyprogesterone acetate (MPA) in combination with estradiol, and with the proliferation of MCF-7, a human breast cancer cell-line, as end-point. Proliferation of MCF-7 cells was measured by means of a crystal violet staining technique. Growth was triggered using a constant estradiol concentration of 10(-10) mol/l, while varying the MPA concentration from 10(-11) to 10(-6) mol/l. The continuous combined model of treatment led to the inhibition of estradiol-induced growth of MCF-7 with MPA concentrations of 10(-10) mol/l and upwards, compared with estradiol-alone-induced growth. The sequential combined model showed a greater inhibition at the higher MPA concentrations of 10(-8)-10(-6) mol/l, with reduced sensitivity to inhibition at the lower MPA concentrations tested, of 10(-11)-10(-9) mol/l. The different treatment types resulted in significantly different sensitivities of the MCF-7 cells to inhibition of estradiol-induced proliferation at the ...Continue Reading

References

Jun 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·B van der BurgS W de Laat
Oct 1, 1989·Analytical Biochemistry·W KuengU Eppenberger
Jun 4, 1988·Lancet·B A Stoll, S Parbhoo
Dec 15, 1988·International Journal of Cancer. Journal International Du Cancer·M Ewertz
Nov 1, 1973·Journal of the National Cancer Institute·H D SouleM Brennan
Nov 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·W H Catherino, V C Jordan
Feb 1, 1995·Journal of the National Cancer Institute·P G TonioloB S Pasternack
Feb 1, 1995·Japanese Journal of Cancer Research : Gann·K HiroseS Tokudome
Jul 3, 1993·Lancet·T J PowlesM O'Brien
Mar 1, 1993·American Journal of Surgery·A G WileD A Margileth
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·W G SchoonenH J Kloosterboer
Jan 1, 1996·American Journal of Obstetrics and Gynecology·J M ClineB von Schoultz
Nov 15, 1995·Cancer·P J DiSaiaW T Creasman
Jun 5, 1998·Annual Review of Public Health·E Barrett-Connor, D Grady
Feb 26, 1999·Journal of the National Cancer Institute·C SchairerR N Hoover
Apr 21, 1999·International Journal of Cancer. Journal International Du Cancer·C MagnussonI Persson
May 21, 1999·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·T E Rohan, A B Miller
Jun 15, 1999·JAMA : the Journal of the American Medical Association·S M GapsturT A Sellers
Sep 11, 1999·Cancer Causes & Control : CCC·I PerssonC Schairer
Oct 20, 1999·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M Dören
Feb 5, 2000·JAMA : the Journal of the American Medical Association·C SchairerR Hoover
Feb 17, 2000·Journal of the National Cancer Institute·R K RossM C Pike
Mar 26, 2002·Climacteric : the Journal of the International Menopause Society·A R Genazzani
Mar 26, 2002·Climacteric : the Journal of the International Menopause Society·L Speroff
Mar 26, 2002·Climacteric : the Journal of the International Menopause Society·B G Wren

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Citations

Jul 9, 2004·The Journal of Obstetrics and Gynaecology Research·Masahiro NozakiHitoo Nakano
Mar 11, 2003·Menopause : the Journal of the North American Menopause Society·UNKNOWN North American Menopause Society
Dec 13, 2003·Maturitas·René Druckmann

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