Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood

Stem Cells International
Gecai ChenLi Zhu

Abstract

Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34(+), cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34(+) count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05).  Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.

References

Jan 30, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Natkunam KetheesanJustin T La Brooy
Feb 3, 2007·American Journal of Hematology·David BerzPeter J Quesenberry
Apr 14, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jaap Jan BoelensUNKNOWN EUROCORD, Inborn error Working Party of EBMT and Duke University
Apr 12, 2011·International Journal of Hematology·Hiroshi YagasakiHideo Mugishima
May 17, 2011·British Journal of Haematology·Tomohiro MorioUNKNOWN Japanese Cord Blood Bank Network
Jan 4, 2013·Biological Research·Francisco BarrigaNicolás Rojas
Jul 9, 2013·Experimental Hematology·Hélène Labussière WalletMauricette Michallet
Oct 8, 2013·Cytotherapy·Alexandra BriquetYves Beguin
Oct 10, 2013·International Journal of Laboratory Hematology·Y B ZhangJ Han
Jun 27, 2015·PloS One·Alireza AbazariMehmet Toner

❮ Previous
Next ❯

Citations

May 24, 2016·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Lucilla LecchiMaurizio Marconi
Apr 30, 2017·Biomedical Reports·Daniela MartinettiLuisa Vicari
Nov 14, 2018·Frontiers in Pharmacology·Gina D KusumaRebecca Lim
Mar 1, 2021·Transfusion Medicine Reviews·Suria JahanNicolas Pineault
Apr 3, 2019·Advanced Science·Rami El AssalUtkan Demirci
Jan 9, 2020·Biomacromolecules·Diana Diaz-DussanRavin Narain

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
density gradient centrifugation

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis