Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies.

Clinical and Diagnostic Laboratory Immunology
K R TamulJ D Folds

Abstract

We compared flow cytometric immunophenotyping results obtained by using the lysed whole blood method of sample preparation with those obtained by using Ficoll-Hypaque-separated cells on 44 consecutive specimens from patients with various hematologic malignancies. When the samples were analyzed as a group, seven antigens (CD2, CD3, CD5, CD11c, CD20, CD22, and CD34) demonstrated significantly different percentages of positively staining cells. When the samples were grouped by disease, results for patients with acute lymphocytic leukemia were discordant for CD22 and HLA-DR and results for patients with hairy cell leukemia were discordant for CD34. Most of the differences, however, were not with antigens critical to the evaluation of the malignancy. Additionally, the most frequent reason for differences in the percentage of positive cells was due to isotype control-based placement of the quadrant markers and not an actual discrepancy in staining. However, analysis of the CD34 antigen yielded eight instances in which staining of Ficoll-Hypaque-separated cells was essentially negative, but a clearly positive population was evident with the lysed preparation. This finding has important implications because of the prognostic significan...Continue Reading

References

Aug 1, 1990·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M J BorowitzW M Crist

❮ Previous
Next ❯

Citations

Jun 16, 2015·American Journal of Respiratory and Critical Care Medicine·Francesca PolverinoCaroline A Owen
Aug 5, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ondřej PelákTomáš Kalina
Nov 23, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Curt I CivinJames C Sturm
Nov 23, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andrew Filby
Nov 17, 2012·Nature Reviews. Immunology·Andrea ValleManuela Battaglia
Nov 22, 2012·European Journal of Haematology·Giovanni RossiNicola Cascavilla
Apr 7, 2009·Current Protocols in Immunology·Ivan J FussHeddy Zola
Aug 29, 2009·Cytometry. Part B, Clinical Cytometry·Mohammad FereidouniMahmoud Mahmoudi
Apr 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Abigail BenitezKimberly J Payne
Jan 17, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Immanuel AndräMatthias Schiemann
Nov 5, 1998·Clinical and Diagnostic Laboratory Immunology·P SchlenkeG Bein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.