Comparison of the effects of insulin, PDGF, interleukin-6, and interferon-gamma on glucose transport in 3T3-L1 cells: lack of cross-talk between tyrosine kinase receptors and JAK/STAT pathways

Diabetologia
C HuppertzH G Joost

Abstract

The effects of insulin, insulin-like growth factor (IGF)-I, platelet-derived growth factor (PDGF), interleukin (IL)-6 and interferon-gamma on 2-deoxyglucose uptake and insulin receptor substrate (IRS)-1 phosphorylation were compared in 3T3-L1 cells at confluence and after differentiation to the adipocyte-like phenotype. Insulin and IGF-I produced the expected stimulation of glucose transport and tyrosine phosphorylation of IRS-1 in both confluent and differentiated cells. In contrast, IL-6 and interferon-gamma failed to stimulate glucose transport or IRS-1 phosphorylation, although a marked stimulation of the JAK/STAT pathways as shown by acute-phase response factor (APRF)/Stat3 or Stat1 activation was observed in fibroblasts (IL-6, interferon-gamma) and adipocytes (IL-6). PDGF-AA and PDGF-BB stimulated glucose transport in confluent, undifferentiated cells to the same extent as insulin (approximately six-fold stimulation), but produced only a small portion of the effect of insulin in differentiated cells. Similarly, mRNA levels and autophosphorylation of PDGF receptors were much lower in differentiated cells than in confluent fibroblasts. In contrast to insulin and IGF-I, PDGF failed to stimulate tyrosine phosphorylation of IR...Continue Reading

Citations

Oct 27, 1998·Journal of Neuroscience Research·P Dell'AlbaniJ de Vellis
Jun 8, 2000·Annals of the New York Academy of Sciences·S A SummersM J Birnbaum
Feb 2, 2008·Blood·Florian GrebienRichard Moriggl
Dec 22, 1998·European Journal of Cell Biology·H Staiger, G Löffler
Dec 2, 2009·Molecular and Cellular Endocrinology·Kalypso Karastergiou, Vidya Mohamed-Ali
Mar 6, 1999·Experimental Cell Research·M YamamotoY Ishikawa
Jul 9, 2004·Diabetologia·A L Carey, M A Febbraio
Oct 3, 1998·The Journal of Biological Chemistry·M YamamotoY Ishikawa
Jun 26, 1999·The Journal of Biological Chemistry·L WangY Ebina
Aug 14, 1999·The Journal of Biological Chemistry·S A SummersM J Birnbaum

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