PMID: 8983418Jan 1, 1996Paper

Comparison of the expression of a plastidic chaperonin 60 in different plant tissues and under photosynthetic and non-photosynthetic conditions

Planta
G SchmitzJ Feierabend

Abstract

A partial cDNA which codes for the beta-subunit of a plastidic chaperonin 60 (cpn60-beta) from rye (Secale cereale L.) leaves was identified and sequenced, except for 46 amino acids of the N-terminus of the mature protein and the transit sequence. This is the first cpn60-beta sequence determined for a monocotyledonous plant. Specific antibodies against cpn60-beta were affinity-purified from an antiserum raised against the total soluble protein fraction of ribosome-deficient plastids. The localization of cpn60-beta in chloroplasts or non-green plastids was confirmed by immunodetection in Percoll gradient-purified organelles. The expression and occurrence of cpn60-beta was analysed by immunoblotting with the specific antibodies and Northern hybridization. The cpn60-beta protein was constitutively expressed in various green and non-green tissues. It was evenly distributed along the major part of a rye leaf, while highest transcript levels occurred in the youngest and oldest leaf sections. The expression of the cpn60-beta protein was not enhanced by a heat-shock treatment at 42 degrees C. The cpn60-beta transcript and protein were more strongly expressed in various non-green, for instance etiolated, 70S-ribosome-deficient 32 degree...Continue Reading

Citations

Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Luigi PerbelliniFranco Tagliaro
Sep 17, 2013·Trends in Plant Science·Anna Vitlin GruberCeleste Weiss
Jan 28, 2006·Journal of Molecular Biology·P WeberA Sokolenko
Jan 18, 2015·Biochimica Et Biophysica Acta·Raphael TröschFelix Willmund

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