Comparison of the functional properties of the monomeric and dimeric forms of the isolated CP47-reaction center complex.

The Journal of Biological Chemistry
M BianchettiJ Barber

Abstract

Chlorophyll fluorescence, thermoluminescence, and EPR spectroscopy have been used to investigate the functional properties of the monomeric and dimeric forms of the photosystem II CP47-reaction center (CP47-RC) subcore complex that was isolated (Zheleva, D., Sharma, J., Panico, M., Morris, H. R., and Barber, J. (1998) J. Biol. Chem. 273, 16122-16127). Chlorophyll fluorescence yield changes induced either by the initiation of continuous actinic light or by repetitive light flashes indicated that the dimeric, but not the monomeric, form of the CP47-RC complex showed secondary electron transport properties indicative of QA reduction. Thermoluminescence measurements also clearly distinguished the monomer from the dimer in that the latter showed a ZV band, which appeared at -55 degreesC, following illumination at -80 degreesC. This band has been determined to be an indicator of the photoaccumulation of QA-. The ability of the dimeric CP47-RC to show secondary electron transport properties was clearly demonstrated by EPR studies. The dimer was characterized by organic radical signals at about g = 2 induced either by illumination or by the addition of dithionite. The dithionite-induced signal was attributed to QA-, but there was no in...Continue Reading

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Citations

Nov 10, 2013·Journal of Photochemistry and Photobiology. B, Biology·S K ZharmukhamedovV V Klimov
Mar 10, 2001·Journal of Photochemistry and Photobiology. B, Biology·J WangN Zhao
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Elmars KrauszSindra Peterson Arsköld
Oct 9, 2001·European Journal of Biochemistry·I SzabòG M Giacometti
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BüchelC Dismukes
Nov 25, 2006·Biochimica Et Biophysica Acta·Hiroshi IshikitaErnst-Walter Knapp
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·K H Rhee
Feb 3, 2016·Plant, Cell & Environment·Dmitriy ShevelaLutz A Eichacker

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