Comparison of the genotypic and phenotypic properties of HIV-1 standard subtype B and subtype B/B' env molecular clones derived from infections in China

Emerging Microbes & Infections
Hui XieYouchun Wang

Abstract

Although a number of standardized human immunodeficiency virus 1 (HIV-1) pseudoviruses have been generated to assess neutralizing antibodies, subtype B/B' has not been comprehensively characterized either genotypically or phenotypically. Full-length env genes were isolated from plasma samples derived from B/B'-infected individuals, including former plasma donors and men who had sex with men. The neutralization properties of these pseudoviruses were determined by testing against sCD4, 16 neutralizing monoclonal antibodies and 44 plasma samples, including samples from those infected with the three major prevalent clades in China. Twenty-eight pseudoviruses were successfully constructed, including 15 B' and 13 B strains. Compared with subtype B strains from North America and Europe, the B' strains from China showed greater variation in the V3 loop and higher susceptibility to the neutralizing antibody 2F5 and B/B' plasma samples. The B' strains from China showed significantly lower susceptibility to some trimer apex-binding neutralizing antibodies (PGT145, CH01, CH02, CH03, and CH04) than the B strains from Western countries. The 28 B-pseudotyped and B'-pseudotyped viruses were grouped into high, medium, and low clusters based on ...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
Dec 1, 1994·AIDS Research and Human Retroviruses·M PurtscherA Jungbauer
Nov 13, 1999·Journal of Virology·J M CrawfordD C Montefiori
Jul 13, 2000·AIDS Research and Human Retroviruses·G Santoro-LopesM Schechter
Mar 21, 2003·Nature·Xiping WeiGeorge M Shaw
Nov 4, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Adriana de BritoJorge Casseb
Jan 23, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Huihui ChongYouchun Wang
Jun 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stanley A Plotkin
Nov 21, 2008·Methods in Molecular Biology·David C Montefiori
Feb 21, 2009·PloS One·Eva Maria FenyöGabriella Scarlatti
Oct 22, 2009·The New England Journal of Medicine·Supachai Rerks-NgarmUNKNOWN MOPH-TAVEG Investigators
Jan 22, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jianhui NieYouchun Wang
Aug 3, 2010·PloS One·Elcio Leal, Fabiola E Villanova
Aug 19, 2011·Nature·Laura M WalkerPascal Poignard
May 29, 2012·The Journal of Infectious Diseases·David C MontefioriJerome H Kim
Dec 3, 2013·Journal of Immunological Methods·Marcella Sarzotti-KelsoeDavid C Montefiori

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Citations

Dec 21, 2019·Emerging Microbes & Infections·Jianhui NieYouchun Wang

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Methods Mentioned

BETA
transfection
glycosylation
PCR

Software Mentioned

GraphPad Prism
Excel
ClustalW
MEGA

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