Comparison of the glucose dependency of glucagon-like peptide-1 (7-37) and glyburide in vitro and in vivo

Metabolism: Clinical and Experimental
D M HargroveJ C Parker

Abstract

The purpose of the present study was to compare the glucose dependency of the insulin secretagogue activity of the sulfonylurea, glyburide, versus that of glucagon-like peptide-1(7-37) [GLP-1(7-37)] in vitro and in vivo. In freshly isolated rat islets, maximally effective concentrations of glyburide (10 micromol/L) and GLP-1(7-37) (10 nmol/L) were equally effective in stimulating insulin secretion in the presence of 15 mmol/L glucose (2.4-fold increase relative to 15 nmol/L glucose alone). At 5 nmol/L glucose, both agents increased insulin secretion, but the effect for glyburide was threefold greater than for GLP-1(7-37) (122% and 41% increase in insulin secretion, respectively). In conscious catheterized rats infused with glucose at a variable rate to clamp plasma glucose concentration at 11 mmol/L, glyburide (1 mg/kg orally) and GLP -1(7-37) (infused intravenously [IV] at 5 pmol/min/kg) produced similar increase in insulin levels (1.8-fold relative to the respective vehicle controls) that were sustained through 60 minutes of measurement. These doses of GLP-1(7-37) and glyburide were then administered to fasted and fed rats (basal plasma glucose concentration, 5.8 and 7.3 mmol/L, respectively). Relative to the vehicle control ...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Thorens
Mar 1, 1992·European Journal of Clinical Investigation·E G SiegelW Creutzfeldt
Apr 1, 1991·The Journal of Endocrinology·A Faulkner, H T Pollock
Mar 1, 1988·The Journal of Endocrinology·R Göke, J M Conlon
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D J DruckerJ F Habener
Sep 1, 1995·Metabolism: Clinical and Experimental·D M HargroveR W Stevenson
Jan 1, 1995·The Journal of Clinical Investigation·G C Weir
Jan 1, 1995·The Journal of Clinical Investigation·Z WangS R Bloom
Jan 1, 1993·Metabolism: Clinical and Experimental·G K HendrickG C Weir
Apr 8, 1993·Regulatory Peptides·W S ZawalichH Rasmussen
Jan 1, 1993·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·R GökeB Göke

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Citations

Mar 11, 2005·Diabetes/metabolism Research and Reviews·Juris J Meier, Michael A Nauck
Feb 28, 2001·Diabetes, Obesity & Metabolism·M J Reed, K A Scribner
Jul 2, 2002·Primates; Journal of Primatology·Koichiro Zamma
Aug 6, 2013·European Journal of Clinical Investigation·Rodrigo A Fraga-SilvaNikos Stergiopulos
Feb 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Susana RavassaJavier Díez

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