Comparison of the human and mouse genes encoding the telomeric protein, TRF1: chromosomal localization, expression and conserved protein domains

Human Molecular Genetics
D BroccoliT de Lange

Abstract

Mammalian chromosome ends contain long arrays of TTAGGG repeats that are complexed to a telomere specific protein, the TTAGGG repeat binding factor, TRF1. Here we describe the characterization of genes encoding the human and mouse TRF1 proteins, hTRF1 and mTRF1. The mTRF1 cDNA was isolated based on sequence similarity to the hTRF1 cDNA and the mTRF1 mRNA was shown to be ubiquitously expressed as a single 1.9 kb polyadenylated transcript in mouse somatic tissues. High levels of a 2.1 kb transcript were found in testes. In vitro translation of the mTRF1 cDNA resulted in a 56 kDa protein that binds to TTAGGG repeat arrays. mTRF1 displayed the same sequence specificity as hTRF1, preferring arrays of TTAGGG repeats as a binding substrate over TTAGGC and TTGGGG repeats. Expression of an epitope-tagged version of mTRF1 showed that the protein is located at the ends of murine metaphase chromosomes. In agreement, conceptual translation indicated that mTRF1 and hTRF1 are similarly-sized proteins with nearly identical C-terminal Myb-related DNA binding motifs. In addition, comparison of the predicted mTRF1 and hTRF1 amino acid sequences showed that the acidic nature of the N-terminus of TRF1 is conserved and revealed a highly conserved no...Continue Reading

Citations

Jul 1, 1997·Somatic Cell and Molecular Genetics·A C YoungA Y Sakaguchi
May 10, 2005·Experimental Cell Research·Nils Hartmann, Harry Scherthan
Feb 1, 2003·Theriogenology·Norikazu MiyashitaTakashi Nagai
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D Kipling
Nov 5, 1997·Nature Genetics·D BroccoliT de Lange
Nov 5, 1997·Nature Genetics·T BilaudE Gilson
Aug 22, 2003·Cloning and Stem Cells·P G Shiels, A G Jardine
Dec 5, 2000·Molecular Biology of the Cell·H ScherthanT de Lange
Oct 2, 2009·Molecular Biology of the Cell·Anneke K BrouwerRoeland W Dirks
Apr 1, 1997·The EMBO Journal·A BianchiT de Lange
Aug 1, 1997·Nucleic Acids Research·G M Coviello-McLaughlin, K R Prowse
May 10, 2006·Nucleic Acids Research·Susan M Bailey, John P Murnane
Feb 12, 2000·Molecular and Cellular Biology·A SmogorzewskaT de Lange
Aug 29, 2003·Molecular and Cellular Biology·Jan KarlsederTitia de Lange
Mar 2, 2006·Molecular and Cellular Biology·Y Jeffrey ChiangRichard J Hodes
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Krauskopf, E H Blackburn
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·L ZhuR J Hodes
Jul 14, 2004·The Journal of Cell Biology·Tatiana Nikitina, Christopher L Woodcock
Apr 17, 2007·Cancer Science·Yukiko MuramatsuHiroyuki Seimiya
Feb 24, 2006·Mutation Research·Alejandro D Bolzán, Martha S Bianchi
Nov 29, 2005·Gene·N CrumetJ Shampay
May 30, 2006·Current Biology : CB·Megan van Overbeek, Titia de Lange
Jun 28, 2016·Molecular Cytogenetics·Galina N PochukalinaAlexey P Voronin
Dec 12, 2012·Molecular Immunology·Asif S QaseemUday Kishore
May 22, 2002·Biology of Reproduction·Norikazu MiyashitaTakashi Nagai
Feb 22, 2017·Antioxidants·Marko IvancichNeelu Puri
Sep 25, 2004·Journal of Cellular Biochemistry·Céline Silva LagesFrançois D Boussin
Feb 20, 1997·Nature·B van Steensel, T de Lange
Nov 16, 2019·Cellular and Molecular Life Sciences : CMLS·Eric M SmithJayakrishnan Nandakumar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.