Comparison of the immune responses associated with experimental bovine mastitis caused by different strains of Escherichia coli

The Journal of Dairy Research
Shlomo BlumGabriel Leitner

Abstract

We studied the mammary immune response to different mammary pathogenic Escherichia coli (MPEC) strains in cows, hypothesising that the dynamics of response would differ. E. coli is a major aetiologic agent of acute clinical bovine mastitis of various degrees of severity with specific strains being associated with persistent infections. We compared challenge with three distinct pathogenic MPEC strains (VL2874, VL2732 and P4), isolated from different forms of mastitis (per-acute, persistent and acute, respectively). A secondary objective was to verify the lack of mammary pathogenicity of an environmental isolate (K71) that is used for comparison against MPEC in genomic and phenotypic studies. Twelve cows were challenged by intra-mammary infusion with one of the strains. Cellular and chemokine responses and bacterial culture follow-up were performed for 35 d. All cows challenged by any of the MPEC strains developed clinical mastitis. Differences were found in the intensity and duration of response, in somatic cell count, secreted cytokines (TNF-α, IL-6 and IL-17) and levels of milk leucocyte membrane Toll-like receptor 4 (TLR4). A sharp decrease of TLR4 on leucocytes was observed concomitantly to peak bacterial counts in milk. Int...Continue Reading

References

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Feb 5, 2013·Veterinary Microbiology·Shlomo E Blum, Gabriel Leitner
Jun 8, 2014·The Veterinary Journal·Shlomo E BlumGabriel Leitner
Sep 4, 2015·PloS One·Shlomo E BlumGabriel Leitner
Jan 26, 2016·PloS One·Florent KempfPierre Germon

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Citations

Nov 5, 2017·Applied and Environmental Microbiology·Michael A OlsonDavid L Erickson
Nov 27, 2018·The Journal of Veterinary Medical Science·Yuya NagasawaTomohito Hayashi
Sep 16, 2020·Animals : an Open Access Journal From MDPI·Muhammad Zahoor KhanZhijun Cao
Apr 14, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Narsingh R Nirala, Giorgi Shtenberg
Oct 17, 2020·Veterinary Research·Pascal RainardGilles Foucras

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