Comparison of the immune responses to the CIA06-adjuvanted human papillomavirus L1 VLP vaccine with those against the licensed HPV vaccine Cervarix™ in mice

Vaccine
Ji Eun HanHong-Jin Kim

Abstract

CIA05 is a toll-like receptor (TLR) 4 agonist derived from an Escherichia coli lipopolysaccharide (LPS) mutant and has been shown to have potential as a vaccine adjuvant. In this study, we investigated the immunopotentiating activity of the adjuvant system CIA06, which is comprised of CIA05 and aluminum hydroxide (alum), when used with the human papillomavirus (HPV) L1 virus-like particles (VLPs) vaccine. BALB/c mice were immunized intramuscularly three times at 2-week intervals with HPV16 L1 VLPs alone or in the presence of various combinations of CIA05 and alum, and the immune responses were assessed. We found that the combination of CIA05 and alum at a ratio of 1:50 (designated CIA06B) yielded the highest immune response in terms of serum anti-HPV L1 VLP IgG antibody titers, splenocyte interferon (IFN)-γ secretion, and antigen-specific memory B cell responses. The immunogenicity of the CIA06B-adjuvanted HPV16/18 L1 VLP vaccine was compared with that of the currently licensed HPV vaccine Cervarix™. The CIA06B-adjuvanted vaccine was similar to Cervarix™ with regard to eliciting serum antigen-specific IgG antibodies and virus-neutralizing antibodies but more effective at inducing splenic cytokine production and memory B cells. ...Continue Reading

References

Apr 18, 1997·The Journal of Biological Chemistry·N QureshiD T Golenbock
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Aug 6, 2003·Expert Review of Vaccines·Jay T EvansJory R Baldridge
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
Jun 9, 2004·Virology·Ethel-Michele de VilliersHarald zur Hausen
Sep 30, 2004·Nature Immunology·Kasper HoebeBruce Beutler
Oct 13, 2007·Expert Review of Vaccines·Sally T Ishizaka, Lynn D Hawkins
Aug 19, 2009·Protein Expression and Purification·Hyoung Jin KimHong-Jin Kim
Oct 31, 2009·International Immunopharmacology·Ji Eun HanNa Gyong Lee

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Citations

Jul 28, 2013·Expert Review of Vaccines·Adele MountAdriana Baz Morelli
Jul 9, 2014·Clinical and Experimental Vaccine Research·Kwang Sung KimYang Je Cho
Jan 27, 2018·Expert Review of Vaccines·William S BowenHaval Shirwan

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