PMID: 9161636Apr 1, 1997Paper

Comparison of the immunobead binding test (IBT) and immunospheres (IS) assay for detecting serum antisperm antibodies

American Journal of Reproductive Immunology : AJRI
G M CentolaA Deutsch

Abstract

The IBT is considered the gold standard of sperm antibody assays. This test uses polyacrylamide beads labeled with antiglobulins (anti-IgG, anti-IgA, and anti-IgM), which bind to the corresponding antibody on the sperm surface. The IS uses color-coded latex beads of uniform 3.0 microns size coated with the antiglobulins which can be viewed with brightfield light microscopy. The purpose of the present study was to compare the IBT and IS in an indirect test using human serum. Serum specimens (n = 42) were tested for the presence of antibody isotypes IgG, IgA, and IgM to sperm using the standard protocol for IBT and IS. Donor sperm was washed in BWW with 5% BSA and diluted to a final concentration of 50 x 10(6) motile sperm/ ml. The sperm were incubated with a 1:10 dilution of test serum for 30 min to 1 h at 37 degrees C and then washed by three cycles of centrifugation. The sperm and beads (IBT, IS) were mixed on a glass slide, covered with a coverslip, and observed within 5 min. At least 100 motile sperm were counted and scored for bead binding. A specimen was considered positive if 20% or more of the sperm were coated with one or more beads. The data were analyzed using calculation of the non-parametric kappa statistic with cor...Continue Reading

References

Feb 1, 1992·Journal of Assisted Reproduction and Genetics·S S WitkinZ Rosenwaks
Dec 1, 1986·Journal of in Vitro Fertilization and Embryo Transfer : IVF·S M JunkJ L Yovich
Jun 1, 1985·American Journal of Reproductive Immunology and Microbiology : AJRIM·G N ClarkeW I Johnston
May 1, 1994·Fertility and Sterility·P B Marshburn, W H Kutteh
May 1, 1964·American Journal of Obstetrics and Gynecology·R R FRANKLIN, C D DUKES

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Citations

Jan 20, 2018·American Journal of Reproductive Immunology : AJRI·Yu WakimotoHiroaki Shibahara
Sep 5, 2020·American Journal of Reproductive Immunology : AJRI·Hiroaki ShibaharaAkiko Hasegawa
Jul 1, 2005·Analytical Chemistry·Joung-Hwan ChoSe-Hwan Paek

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