Comparison of the immunoglobulin heavy-chain complementarity determining region-3 structure among the DNA sequences and the mu- and gamma-transcripts in human B-lineage cells

Immunology
H KiyoiT Naoe

Abstract

To study the recombinational significance of the (immunoglobulin heavy) IgH chain gene in human B-cell development, we compared the complementarity determining region (CDR)-3 sequences of the DNA and the mu-transcripts from human normal pre-B cells and mature B cells, and the gamma-transcripts from bone marrow cells. The CDR-3 sequences were longer in the DNA than in the mu- and gamma-transcripts, and this was independent of whether or not the rearrangement was productive. The DLR family genes were less frequently used in the mu- and gamma-transcripts. When translated into amino acids, all CDR-3 sequences from the mu- and gamma-transcripts were productive, although 26.2% of the DNA sequences had stop codons in the D element and/or frameshifts of the JH gene segments. The CDR-3 of the productive DNA sequences in pre-B cells frequently (26.6%) contained at least three continuous hydrophobic amino acids, which were mainly coded by the DLR and DXP family genes at the third reading frame. However, such motifs were rate in the mu-transcripts of pre-B (7.7%) and mature B cells (3.9%), and in the gamma-transcripts of bone marrow cells (1.1%) as well as in the DNA of mature B cells (10.4%). These findings suggested that the length and/o...Continue Reading

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Citations

May 25, 2001·International Journal of Hematology·H Kiyoi, T Naoe
Jul 30, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Federico Caligaris-Cappio, Paolo Ghia
Jul 26, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Takaomi C Saido
Jul 15, 2015·Journal of Speech, Language, and Hearing Research : JSLHR·Meital Avivi-ReichBruce A Schneider
Jun 28, 2003·American Journal of Speech-language Pathology·Nina N Simmons-Mackie, Jack S Damico

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