Comparison of the in vitro characteristics of von Willebrand factor in British and commercial factor VIII concentrates

British Journal of Haematology
A S LawrieG F Savidge

Abstract

Qualitative/quantitative analysis of von Willebrand factor antigen (vWf:Ag) in either heat or solvent/detergent treated factor VIII concentrates, used for haemophilia replacement therapy, was undertaken to assess their suitability for the treatment of vWD. For the first time immunoaffinity purified vWf:Ag (Monoclate by-product) was also evaluated by in vitro assessment. Potencies of vWf:Ag varied considerably but were consistently higher (28.9-420.5 iu/ml) than factor VIII:C (one-stage) activity (8.13-42.44 iu/ml). The functional activity of vWf was assessed by either Ristocetin Cofactor (vWf:RCo) or collagen binding methods (vWf:CBA) with typical vWf:RCo/vWf:Ag ratios ranging from 0.08 to 0.94. Multimeric analysis confirmed that in vitro biological activity was dependent on the presence of the high molecular weight forms of vWf:Ag. A significant correlation (r = 0.95) between vWf:RCo activity and collagen binding was observed in all of the concentrates with the exception of the immunopurified product. The data suggest that either NHS 8Y (mean vWfRCo/vWf:Ag = 0.94), Haemate P (mean vWf:RCo/vWf:Ag = 0.69) and high purity Octapharma V.I (vWf:RCo/vWf:Ag = 0.82) which contain medium/high MW vWf:Ag multimers are likely to be most co...Continue Reading

References

Nov 1, 1978·The Journal of Clinical Investigation·J Lazarchick, L W Hoyer
Oct 1, 1977·British Journal of Haematology·R J Evans, D E Austen
Aug 1, 1986·Thrombosis Research·J E Brown, J O Bosak
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·S Rosén

❮ Previous
Next ❯

Citations

Feb 13, 2001·British Journal of Haematology·S L AllfordS J Machin
Feb 24, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·A DobrkovskaJ R Chediak
Apr 1, 1992·British Journal of Haematology·T FaconJ Goudemand
Sep 17, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·W Schramm
May 4, 2011·Haemophilia : the Official Journal of the World Federation of Hemophilia·J C GillM Manco-Johnson
May 1, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·E Berntorp
Mar 21, 1997·The Journal of Biological Chemistry·M O PentikäinenP T Kovanen
Jan 19, 2019·Research and Practice in Thrombosis and Haemostasis·Anne RiddellTom McKinnon
Oct 1, 1990·La Ricerca in Clinica E in Laboratorio·P M MannucciM Cattaneo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.