Comparison of the in vitro hemolytic effects produced by alkoxyacetic acids on human and rat erythrocytes

International Journal of Occupational Medicine and Environmental Health
Andrzej StarekWiesław Szymczak

Abstract

The alkoxyacetic acids (AAAs) are urinary metabolites of alkoxyethanol solvents. It is well documented that these chemicals can cause acute hemolytic anemia in humans and laboratory animals. There are scarce data on the relative hemolytic activity of these acids. Likewise, information is lacking on the relationship between their hemolytic activity and physicochemical properties. The aim of this study was to compare the hemolytic activity of five AAAs in red blood cells (RBCs) derived from donors' blood and male Wistar rats. Moreover, the possible relationship between lipophilic and hemolytic activity of AAAs was also investigated. The RBCs washed in TRIS buffer, pH 7.4, were adjusted to a packed cell volume (PCV) of about 20% and incubated in a water bath at 37 degrees C for 0-3 h in the presence of different concentrations of AAAs. The hemolytic effects, in terms of the changes in RBCs, PCV, mean corpuscular volume (MCV) and free hemoglobin (HGBfree) in incubation medium, were evaluated. Based on the dose-response relationship for RBCs, PCV and MCV, the effective concentration values (EC50) and their 95% confidence intervals (95% CI) were calculated. The octanol-water partition coefficient (log P) and distribution coefficient ...Continue Reading

References

Mar 1, 1988·Human Toxicology·M O Rambourg-SchepensD Lamiable
Jan 1, 1987·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·F G BartnikK Kunstler
Jul 1, 1985·Toxicology and Applied Pharmacology·E J MossT J Gray
Aug 1, 1984·Environmental Health Perspectives·B K NelsonP T Goad
Aug 1, 1984·Environmental Health Perspectives·B D HardinJ R Burg
Jan 1, 1997·International Archives of Occupational and Environmental Health·V HaufroidD Lison
Jul 13, 2000·Human & Experimental Toxicology·B I GhanayemA Nyska
Sep 7, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mark M Udden
Jan 30, 2003·Occupational and Environmental Medicine·T-S ShihS-H Liou
May 28, 2004·International Archives of Occupational and Environmental Health·E Ben-BrikLuc Multigner
Nov 20, 2004·Archives of Toxicology·David J LockleyFaith M Williams
Feb 12, 2005·Toxicology Letters·P de Ketttenis
Feb 12, 2005·Toxicology Letters·A FastierD B McGregor

❮ Previous
Next ❯

Citations

Aug 3, 2014·Neurotoxicity Research·Bartosz PomiernyBogusława Budziszewska
May 15, 2012·Pharmacological Reports : PR·Beata Starek-ŚwiechowiczAndrzej Starek
Aug 9, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Bartosz PomiernyBogusława Budziszewska
Nov 3, 2010·Toxicology and Applied Pharmacology·Patricia RuizVijay Gombar
Jun 13, 2012·The Journal of Toxicological Sciences·Takashi YamadaMakoto Hayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.