Comparison of the in vitro toxicity of indocyanine green to that of trypan blue in human retinal pigment epithelium cell cultures

American Journal of Ophthalmology
Jeffrey S GaleCindy M L Hutnik

Abstract

To compare the in vitro toxicity of indocyanine green (ICG) to that of trypan blue (TB) in human retinal pigment epithelium cell cultures. The use of ICG and TB in macular hole surgery is discussed. In vitro cell biology experimental study. The ICG dye and TB were applied to ARPE-19, a commercially available human retinal pigment epithelium cell line. Cultures were established and maintained according to supplier protocols. The ICG dye, TB or Hank's balanced salt solution (controls) were then applied to the cells at varying concentrations and over various exposure periods. Fiberoptic light was also applied to cells to assess for the possibility of a potentiating phototoxic effect. Cell viability fractions were determined using a well-studied mitochondrial dehydrogenase assay. The TB was not toxic to the retinal pigment epithelium cell cultures at any concentration or over any period of exposure, whereas ICG dye demonstrated dose-dependent and exposure-dependent toxicity. The ICG dye was found to be toxic to the cells at all tested concentrations between 5.0 mg/ml (stock concentration, 26.1% cell survival) and 0.5 mg/ml (92.8% cell survival) over a 3-minute exposure. No toxicity to TB was seen at the stock concentration of 1.5 m...Continue Reading

References

Apr 30, 1998·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·E M MessmerA Kampik
Sep 20, 2000·Archives of Dermatological Research·C AbelsR M Szeimies
Jul 27, 2001·Ophthalmology·W E SmiddyG Cordahi
Aug 3, 2001·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·A W WeinbergerN F Schrage
Sep 1, 2001·American Journal of Ophthalmology·A GandorferA Kampik
Dec 31, 2002·Investigative Ophthalmology & Visual Science·Arnd GandorferAnselm Kampik
Feb 5, 2003·American Journal of Ophthalmology·Jau-Der HoHung-Chiao Chen
Dec 24, 2003·Investigative Ophthalmology & Visual Science·Salvatore GrisantiKarl Ulrich Bartz-Schmidt

❮ Previous
Next ❯

Citations

Jul 13, 2006·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·F RüferJ Roider
Apr 19, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·L KodjikianJ G Garweg
May 18, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Stanislao RizzoFederica Cresti
Feb 13, 2010·Eye·S Y Lesnik Oberstein, M D de Smet
Mar 8, 2007·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Hiroshi KunikataKohji Nishida
Apr 11, 2007·Retina·Akifumi UenoTatsuro Ishibashi
Jul 14, 2012·Retina·Andrzej Grzybowski, Francisco J Ascaso
Mar 19, 2005·The British Journal of Ophthalmology·T L Jackson
Jun 20, 2007·The British Journal of Ophthalmology·Sarit Y Lesnik ObersteinMarc D de Smet
Aug 22, 2007·The British Journal of Ophthalmology·Nanny Collaer, Peter Stalmans
Feb 14, 2012·The British Journal of Ophthalmology·Bilal HajnajeebUrsula Schmidt-Erfurth
Feb 24, 2006·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Hiroshi KunikataMakoto Tamai
Nov 27, 2009·BMC Research Notes·S BalaiyaKv Chalam
Feb 11, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Fabrizio GiansantiUgo Menchini
Apr 29, 2014·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Matthew P SimunovicI-Van Ho
Apr 9, 2013·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Claudine BelleriveMarc Hébert
Aug 18, 2009·Survey of Ophthalmology·Eduardo B RodriguesMichel E Farah
Oct 9, 2007·Experimental Eye Research·Swaantje PetersSalvatore Grisanti
Nov 25, 2006·Ophthalmology·Timothy L JacksonWilliam Aylward
May 18, 2006·Acta Ophthalmologica Scandinavica·Wico W LaiDennis S C Lam
Mar 28, 2006·Journal of Cataract and Refractive Surgery·Richard C AllenThomas A Oetting
Sep 12, 2015·ACS Applied Materials & Interfaces·David C YeoHan Wei Hou
Feb 3, 2018·International Ophthalmology·Samir Mohamed El-Baha, Mahmoud Alaa Abouhussein
Mar 1, 2019·Investigative Ophthalmology & Visual Science·David TroiloLyndon Jones
Oct 28, 2010·Experimental Brain Research·Benjamin R KunzWilliam B Thompson
Apr 10, 2008·Retina·André M V GomesWalter Y Takahashi
Mar 5, 2011·The International Journal of Artificial Organs·Yvonne KohlRobert Lemor
Jan 15, 2021·SN Applied Sciences·Tatianna MarshallErica Pensini
Aug 16, 2021·Journal of Microbiological Methods·Marissa A Lopez-PierJohn P Konhilas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.