PMID: 20650065Feb 1, 1995Paper

Comparison of the in vitro toxicity of dichloroaniline structural isomers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M A ValentovicG O Rankin

Abstract

Acute exposure to certain dichloroaniline (DCA) isomers results in renal and hepatic toxicity in vivo. In the present study we examined whether dichloroaniline structural isomers were cytotoxic to liver and kidney slices in vitro and compared the toxicities of the different structural isomers. These studies were necessary in order to validate the use of an in vitro slice system for examination of the cellular mechanisms for toxicity. Renal cortical and hepatic slices were incubated for 90 min with 2,3-DCA, 2,4-DCA, 2,5-DCA, 2,6-DCA, 3,4-DCA or 3,5-DCA at a final concentration of 0-1 mm. Pyruvate-directed gluconeogenesis was measured following an additional 30-min incubation with 10 mM pyruvate. Cytotoxicity was also determined by measurement of lactate dehydrogenase (LDH) release 120 min after the addition of dichloroaniline isomers at a final concentration of 0, 0.5, 1 or 2 mM. Gluconeogenesis in renal cortical slices was inhibited by all of the isomers beginning at a concentration of 0.5 mM. Renal slice LDH leakage was elevated above control levels by 1-2 mM 3,4-DCA or 3,5-DCA. A final concentration of 2 mM was needed for 2,3-DCA, 2,4-DCA, 2,5-DCA or 2,6-DCA in order to detect a significant (P < 0.05) increase in renal slice ...Continue Reading

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Citations

Aug 7, 1998·Chemosphere·L GuilherminoM C Lopes
Nov 25, 2000·Toxicology·C Lerche-Langrand, H J Toutain
Feb 11, 1998·Toxicology·M A ValentovicG O Rankin
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BlackmoreT J Gray
Jan 10, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P OlingaG M Groothuis
Jan 25, 2008·Environmental Toxicology·Gary O RankinJohn B Barnett
Sep 1, 2015·Acta Crystallographica. Section C, Structural Chemistry·Valeska Gerhardt, Michael Bolte
Apr 16, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Xianai WuHans-Joachim Lehmler
Jun 1, 2017·International Journal of Molecular Sciences·Gary O RankinMonica A Valentovic
Nov 18, 2014·International Journal of Molecular Sciences·Christopher RacineGary O Rankin
May 18, 2020·Environmental Toxicology and Pharmacology·Ali Mustafa MohammedKirsi H Vähäkangas

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