Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells

Mutation Research
Takamitsu A KatoJ S Bedford

Abstract

The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the gamma-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the gamma-H2AX assay which depends on extensive phosphorylation and dephosphorylation of the H2AX histone variant surrounding DSBs. The disappearance of gamma-H2AX foci after irradiation was much slower for mitotic than for G1 cells. On the other hand, no difference was seen for the gel electrophoresis assay. Our data may suggest the limited accessibility of dephosphorylation enzyme in irradiated metaphase cells or trapped gamma-H2AX in condensed chromatin.

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Citations

Dec 11, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Laura Lafon-HughesGustavo A Folle
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Teresa NeumaierSylvain V Costes
Sep 6, 2008·Nucleic Acids Research·Andrea KinnerGeorge Iliakis
May 9, 2009·PLoS Genetics·Raghuvar Dronamraju, James M Mason
Jul 25, 2013·International Journal of Molecular Sciences·Maria MoroniChristophe E Redon
Dec 10, 2009·International Journal of Radiation Biology·Penny Jeggo, Martin F Lavin
Aug 24, 2016·European Heart Journal Cardiovascular Imaging·M A HillW G McKenna
Feb 18, 2010·Mutagenesis·Darragh G McArtGillian R Wasson
Dec 23, 2016·Nature Cell Biology·Nicole Hustedt, Daniel Durocher
Feb 12, 2009·Journal of Cellular Physiology·Takamitsu A KatoJoel S Bedford
Mar 10, 2010·Environmental and Molecular Mutagenesis·James D Tucker
Jul 28, 2010·The Journal of Cell Biology·Simona GiuntaStephen P Jackson
Feb 26, 2020·Genes·Sara OvejeroMaría P Sacristán
Sep 22, 2011·International Journal of Cancer. Journal International Du Cancer·Ryuichi Okayasu
Jan 21, 2021·The EMBO Journal·Shibo LiXiaohua Wu

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