PMID: 2116771Aug 15, 1990Paper

Comparison of the kinetics of reduction and intramolecular electron transfer in electrostatic and covalent complexes of ferredoxin-NADP+ reductase and flavodoxin from Anabaena PCC 7119

Archives of Biochemistry and Biophysics
M C WalkerG Tollin

Abstract

The kinetics of reduction and intracomplex electron transfer in electrostatically stabilized and covalently crosslinked complexes between ferredoxin-NADP+ reductase (FNR) and flavodoxin (Fld) from the cyanobacterium Anabaena PCC 7119 were compared using laser flash photolysis. The second-order rate constant for reduction by 5-deazariboflavin semiquinone (dRfH) of FNR within the electrostatically stabilized complex at 10 mM ionic strength (4.0 X 10(8) M-1 s-1) was identical to that for free FNR. This suggests that the FAD cofactor of FNR is not sterically hindered upon complex formation. A lower limit of approximately 7000 s-1 was estimated for the first-order rate constant for intracomplex electron transfer from FNRred to Fldox under these conditions. In contrast, for the covalently crosslinked complex, a smaller second-order rate constant (2.1 X 10(8) M-1 s-1) was obtained for the reduction of FNR by dRfH within the complex, suggesting that some steric hindrance of the FAD cofactor of FNR occurs due to crosslinking. A limiting rate constant of 1000 s-1 for the intracomplex electron transfer reaction was obtained for the covalent complex, which was unaffected by changes in ionic strength. The substantially diminished limiting r...Continue Reading

References

Aug 15, 1989·European Journal of Biochemistry·J J PueyoC Gómez-Moreno
Oct 3, 1989·Biochemistry·L Z MorandD J Davis
Jul 29, 1983·Biochimica Et Biophysica Acta·L M GerenF Millett
Apr 13, 1981·Biochimica Et Biophysica Acta·J M SmithD B Knaff
Sep 1, 1988·Photosynthesis Research·R PschornA Wild

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Citations

Feb 1, 1994·Journal of Bioenergetics and Biomembranes·H M HoldenJ L Markley
Jan 1, 1996·Biochimie·I Sevrioukova, J A Peterson
Jun 1, 1991·Photochemistry and Photobiology·M A Cusanovich
May 15, 1991·Archives of Biochemistry and Biophysics·G Tollin, J T Hazzard
Oct 18, 2002·European Journal of Biochemistry·Merche FaroMilagros Medina
May 18, 1994·Biochimica Et Biophysica Acta·M HirasawaD B Knaff

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