Comparison of the luminescent ADP-Glo assay to a standard radiometric assay for measurement of protein kinase activity

Assay and Drug Development Technologies
Jasbinder SangheraJun Yan

Abstract

Many assay technologies have been developed and utilized to efficiently assay and screen against protein kinase targets. The radiometric assay format for assaying the protein kinase targets has been considered the "Gold Standard" format since it allows the direct readout of kinase functional activity and is a universal assay that is highly sensitive. However, the hazardous nature of the radiometric assay together with the regulatory hurdles has led to the development of alternative assay formats for assessing protein kinase activity measurements. The luminescent ADP-Glo assay has been developed as an alternative to radiometric format for assaying protein kinase targets. This assay allows the measurement of the ADP product formed during the kinase reaction. Therefore, the luminescent ADP-Glo assay is similar to the radiometric format in that it measures the direct product of the protein kinase reaction. Furthermore, since the ADP product is generated by all protein kinase reactions, this is a universal format that can be used for assaying any given protein kinase target. Analysis of data generated with multiple protein kinase targets and the luminescent ADP-Glo technology shows comparable results to the radiometric assay format....Continue Reading

References

Dec 31, 1997·Analytical Biochemistry·R Seethala, R Menzel
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Sep 22, 2000·The Biochemical Journal·S P DaviesP Cohen
Jul 18, 2002·Nature Reviews. Drug Discovery·Philip Cohen
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Apr 20, 2004·Assay and Drug Development Technologies·Steven M RodemsBrian A Pollok
May 29, 2004·Assay and Drug Development Technologies·Mitsunori Koresawa, Takayoshi Okabe

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Methods Mentioned

BETA
protein kinase assay
Assay

Software Mentioned

SignalChem

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