Comparison of the media for isolation and storage of round spermatid nuclei before intracytoplasmic injection

Journal of Assisted Reproduction and Genetics
K SuzukiRyuzo Yanagimachi

Abstract

This study investigated whether K(+)-rich medium is better than pure NaCl solution or Na(+)-rich cell culture medium for handling round spermatid nuclei prior to injection into oocytes (ROSNI). Round spermatids of the mouse were isolated and stored in isotonic NaCl, a cell culture medium (CZB), or a nucleus isolation medium (NIM) before injection into oocytes. The rates of normal fertilization, embryonic development in vitro, and birth of normal offspring after transfer of embryos to foster mothers were determined. In vitro development of ROSNI-produced zygotes to blastocysts was the same when "naked" spermatid nuclei were exposed briefly to three media. However, a long (60-min) exposure of the nuclei to Na(+)-rich medium was detrimental. In K(+)-rich NIM "naked" spermatid nuclei best retained their ability to participate in normal embryonic development. NIM was better than Na(+)-rich medium for retaining isolated spermatids competent to participate in normal embryonic development.

Citations

Feb 19, 2009·Zygote : the Biology of Gametes and Early Embryos·Hiroyuki YazawaAkira Sato
Jun 22, 2000·Biology of Reproduction·H TatenoR Yanagimachi
Mar 6, 2009·Microscopy Research and Technique·Zuping HeMartin Dym
Oct 24, 2001·Biology of Reproduction·J CozziJ P Wolf
Mar 4, 2008·Archives of Andrology·Jonathan M RielMonika A Ward
Feb 10, 2017·Reproductive Biology and Endocrinology : RB&E·Fiammetta BerlinguerEraldo Sanna Passino

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