Comparison of the membrane subproteomes during growth of a new pseudomonas strain on lysogeny broth medium, glucose, and phenol

Journal of Proteome Research
D G PapasotiriouGeorgios Tsiotis

Abstract

Study of the bacterial membrane proteome is a field of growing interest in the research of nutrient transport and processing. Pseudomonas sp. strain phDV1, a Gram-negative bacterium selected for its ability to degrade aromatic compounds, was monitored under different growth substrate conditions, using lysogeny broth medium (LB), glucose, and phenol as sole carbon source. The aim of this study was to characterize the membrane subproteomes of the Pseudomonas strain by proteomic means to assess the protein composition of this subcellular compartments, which appears fundamental for the biodegradation of aromatic compounds. A total number of 129 different proteins have been identified by MALDI-TOF/TOF, 19 of which are membrane proteins that belong to the inner membrane and 10 that belong to the outer membrane. Two membrane proteins were only expressed in the presence of the aromatic substrate. We identified a membrane protein involved in aromatic hydrocarbon degradation as well as a probable porin which may, in fact, function as an aromatic compound-specific porin. Although the presence of different transporters have been reported for different aromatic compounds such as toluene and benzoic acid, to our knowledge, these are the firs...Continue Reading

References

Mar 1, 1986·Journal of Bacteriology·R BenzG H Vos-Scheperkeuter
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Jan 1, 1986·Annual Review of Cell Biology·C Lee, J Beckwith
May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
Jan 1, 1995·Molecular Membrane Biology·M P Sheetz
Jan 14, 1999·Nature Structural Biology·S K BuchananJ Deisenhofer
Apr 29, 2000·Electrophoresis·V SantoniT Rabilloud
Jul 27, 2002·Annual Review of Microbiology·Juan L RamosAna Segura
Feb 26, 2003·Proteomics·Pierre LescuyerThierry Rabilloud
Mar 1, 2003·Nature Biotechnology·Christine C Wu, John R Yates
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Christopher D PivettiMilton H Saier
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Jun 25, 2004·Nucleic Acids Research·Pantelis G BagosStavros J Hamodrakas
Oct 19, 2004·Electrophoresis·Michalis AivaliotisGeorgios Tsiotis
May 4, 2005·Biodegradation·Paraskevi N Polymenakou, Euripides G Stephanou
Apr 5, 2007·Nature Protocols·Hermann Schägger
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann

❮ Previous
Next ❯

Citations

May 14, 2011·Journal of Proteomics·Anastasia PapadiotiGeorgios Tsiotis
May 6, 2009·Current Opinion in Microbiology·Seong-Jae KimCarl E Cerniglia
Jun 12, 2009·Electrophoresis·Thierry Rabilloud
Feb 16, 2010·Journal of Proteomics·Catarina Roma-RodriguesIsabel Sá-Correia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.