PMID: 3285Mar 1, 1976

Comparison of the metabolism of benzo[alpha]pyrene and binding to DNA caused by rat liver nuclei and microsomes

Chemico-biological Interactions
K AlexandrovM H Thompson


Administration of 3-methylcholanthrene (3MC) to rats greatly enhanced the aryl hydrocarbon hydroxylase (AHH) activity of liver nuclei. However, the binding in vitro [3H]benzo[alpha]pyrene (BP) to DNA within the nuclei which occurred at the same time as hydroxylation of BP was much less enhanced. Thin layer chromatography of the metabolites of BP produced by these nuclei revealed the same metabolites in similar relative amounts as were produced by rat liver microsomes prepared from rats which had received 3MC. The binding to DNA was further analysed by hydrolysis of the DNA and fractionation on a Sephadex column. This analysis revealed that the binding to DAN in nuclei was very similar in nature to that which occurred when calf-thymus DNA was added to microsomes metabolising BP.


Dec 1, 1978·Chemico-biological Interactions·A EastmanE Bresnick
Apr 1, 1979·Chemico-biological Interactions·T AuneE Dybing
Jan 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K AlexandrovC Frayssinet
Jul 1, 1977·Journal of Toxicology and Environmental Health·P CeruttiJ Remsen
Nov 11, 1981·Nucleic Acids Research·P L Jack, P Brookes
Jan 1, 1983·Drug Metabolism Reviews·M RomanoM Salmona
Sep 15, 1976·International Journal of Cancer. Journal International Du Cancer·H W KingP Brookes
Dec 14, 1978·Biochemical and Biophysical Research Communications·W E FahlC R Jefcoate
Mar 1, 1978·Chemico-biological Interactions·B JernströmS Orrenius
Jun 15, 1980·Toxicology and Applied Pharmacology·A E Chin, H B Bosmann
Aug 1, 1977·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K Alexandrov
Jun 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P M DansetteC Frayssinet
Jan 1, 1979·Chemico-biological Interactions·E BresnickW Bornstein
Dec 14, 1979·Biochemical and Biophysical Research Communications·T M GuenthnerS Orrenius
Jan 1, 1979·Drug Metabolism Reviews·E Bresnick
Oct 1, 1980·Environmental Research·I D CapelD C Williams
May 23, 1976·Biochemical and Biophysical Research Communications·C L Jahn, G W Litman
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
Sep 15, 1978·International Journal of Cancer. Journal International Du Cancer·G DoerjerP Kleihues

Related Concepts

Xenobiotic Monooxygenases
DNA, Double-Stranded
Electron Microscopy
Microsomes, Liver
Rats, Laboratory

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.