PMID: 3285Mar 1, 1976

Comparison of the metabolism of benzo[alpha]pyrene and binding to DNA caused by rat liver nuclei and microsomes

Chemico-biological Interactions
K AlexandrovM H Thompson

Abstract

Administration of 3-methylcholanthrene (3MC) to rats greatly enhanced the aryl hydrocarbon hydroxylase (AHH) activity of liver nuclei. However, the binding in vitro [3H]benzo[alpha]pyrene (BP) to DNA within the nuclei which occurred at the same time as hydroxylation of BP was much less enhanced. Thin layer chromatography of the metabolites of BP produced by these nuclei revealed the same metabolites in similar relative amounts as were produced by rat liver microsomes prepared from rats which had received 3MC. The binding to DNA was further analysed by hydrolysis of the DNA and fractionation on a Sephadex column. This analysis revealed that the binding to DAN in nuclei was very similar in nature to that which occurred when calf-thymus DNA was added to microsomes metabolising BP.

Citations

Dec 1, 1978·Chemico-biological Interactions·A EastmanE Bresnick
Apr 1, 1979·Chemico-biological Interactions·T AuneE Dybing
Jan 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K AlexandrovC Frayssinet
Jul 1, 1977·Journal of Toxicology and Environmental Health·P CeruttiJ Remsen
Nov 11, 1981·Nucleic Acids Research·P L Jack, P Brookes
Jan 1, 1983·Drug Metabolism Reviews·M RomanoM Salmona
Sep 15, 1976·International Journal of Cancer. Journal International Du Cancer·H W KingP Brookes
Dec 14, 1978·Biochemical and Biophysical Research Communications·W E FahlC R Jefcoate
Mar 1, 1978·Chemico-biological Interactions·B JernströmS Orrenius
Jun 15, 1980·Toxicology and Applied Pharmacology·A E Chin, H B Bosmann
Aug 1, 1977·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K Alexandrov
Jun 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P M DansetteC Frayssinet
Jan 1, 1979·Chemico-biological Interactions·E BresnickW Bornstein
Dec 14, 1979·Biochemical and Biophysical Research Communications·T M GuenthnerS Orrenius
Jan 1, 1979·Drug Metabolism Reviews·E Bresnick
Oct 1, 1980·Environmental Research·I D CapelD C Williams
May 23, 1976·Biochemical and Biophysical Research Communications·C L Jahn, G W Litman
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
Sep 15, 1978·International Journal of Cancer. Journal International Du Cancer·G DoerjerP Kleihues

Related Concepts

Metazoa
Xenobiotic Monooxygenases
Benzpyrene
DNA, Double-Stranded
Liver
Methylcholanthrene
Electron Microscopy
Microsomes, Liver
NADP
Rats, Laboratory

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