PMID: 2496982Apr 15, 1989Paper

Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16

European Journal of Biochemistry
C ZaboroschH Kratzin

Abstract

The cytoplasmic, NAD-linked hydrogenase of the Gram-positive hydrogen-oxidizing bacterium Nocardia opaca 1b was compared with the analogous enzyme isolated from the Gram-negative bacterium Alcaligenes eutrophus H16. The hydrogenase of N. opaca 1b was purified by a new procedure applying chromatography on phenyl-Sepharose and DEAE-Sephacel with two columns in series. A homogeneous enzyme preparation with a specific activity of 74 mumol H2 oxidized.min-1.mg protein-1 and a yield of 32% was isolated. The A. eutrophus enzyme was purified as previously published. Both enzymes are tetrameric proteins composed of four non-identical subunits (alpha, beta, gamma, delta). The four subunits of both of these enzymes were separated and isolated as single polypeptides by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Immunological comparison of the four subunits of the Nocardia hydrogenase with those of the Alcaligenes enzyme showed that the alpha, beta, gamma, and delta subunits of one organism were serologically related to the analogous subunits of the other organism. Among themselves, the four subunits do not have any serological relationship. The eight individual polypeptides were also compared ...Continue Reading

References

Nov 8, 1976·Biochimica Et Biophysica Acta·K Schneider, H G Schlegel
Apr 1, 1977·Archives of Microbiology·K Schneider, H G Schlegel
Dec 16, 1987·Biochemical and Biophysical Research Communications·B C PrickrilJ LeGall
Aug 1, 1983·The Biochemical Journal·K SchneiderK Jochim
Mar 1, 1981·Journal of Bacteriology·B FriedrichC G Friedrich

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Citations

Dec 1, 1990·FEMS Microbiology Reviews·J N Reeve, G S Beckler
Apr 1, 1997·Microbiology·Claudia GrzeszikHans G Schlegel
Jun 5, 1998·Microbiology·Jutta KalkusHans G Schlegel
Dec 30, 1994·Biochimica Et Biophysica Acta·S P Albracht

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