Comparison of the ocular microbiome between chronic Stevens-Johnson syndrome patients and healthy subjects.

Scientific Reports
Thanachaporn KittipibulTanittha Chatsuwan

Abstract

Stevens - Johnson syndrome (SJS) has manifestation through the exfoliation of epidermis and mucosal tissue. Ocular surface is usually affected in acute and chronic stage. The patients are usually suffered from chronic ocular sequelae including symblepharon, limbal stem cell deficiency, etc. Furthermore, ocular microbiome may also be altered in SJS. This is prospective, age and sex matched analytical study which including 20 chronic SJS patients and 20 healthy subjects for specimen collection from inferior conjunctiva for microbiome analysis by conventional cultures and Next-Generation Sequencing (NGS) methods. Significant higher proportion of positive-cultured specimen was demonstrated in SJS group (SJS group 60%, healthy 10%, p-value = 0.001). In addition, NGS which providing high-throughput sequencing has demonstrated the greater diversity of microbial species. The higher proportion of pathogenic microorganisms including Pseudomonas spp., Staphylococcus spp., Streptococcus spp., Acinetobacter spp. was shown in SJS group. Ocular surface in SJS is usually occupied by more diverse microorganisms with increased proportion of pathogenic species. This condition may affect chronic inflammation and opportunistic infections in SJS gro...Continue Reading

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Citations

Dec 17, 2020·Asia-Pacific Journal of Ophthalmology·José Álvaro P GomesVanessa F Demeda
Dec 29, 2020·Experimental Eye Research·Sisinthy ShivajiSomasheila I Murthy
Feb 8, 2021·Experimental Eye Research·Konduri RanjithSisinthy Shivaji
Mar 3, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Pratima VishwakarmaSrikant K Sahu
May 25, 2021·Frontiers in Medicine·Thanachaporn Kittipibul, Vilavun Puangsricharern
Jul 6, 2021·Frontiers in Medicine·Tais Hitomi WakamatsuJosé Álvaro Pereira Gomes
Jul 3, 2021·International Journal of Molecular Sciences·Yoko OgawaKazuo Tsubota
Aug 14, 2021·Current Opinion in Allergy and Clinical Immunology·Lauren ValentineLeonard Bielory
Sep 16, 2021·Investigative Ophthalmology & Visual Science·Zhang ZhangHaidong Zou

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Methods Mentioned

BETA
Illumina sequencing
PCR

Software Mentioned

SUMACLUST
SortMeRNA
FASTX
- Toolkit
QIIME
vsearch1

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