Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes

BioMed Research International
Jaewang LeeSeok Hyun Kim

Abstract

In vitro follicle growth (IVFG) is an emerging fertility preservation technique, which can obtain fertilizable oocytes from an in vitro culture system in female. This study aimed to compare efficiency of the most widely used two-dimensional follicle culture methods [with or without oil layer (O+ or O- group)]. Preantral follicles were isolated from mice and randomly assigned. Follicles were cultured for 10 days and cumulus-oocyte complexes harvested 16-18 hours after hCG treatment. Follicle and oocyte growth, hormones in spent medium, meiotic spindle localization, expression of reactive oxygen species (ROS), mitochondrial activity, and gene expression were evaluated. In follicle growth, survival, pseudoantral cavity formation, ovulation, and oocyte maturation were also significantly higher in O+ group than O- group. Hormone production was significantly higher in follicles cultured in O+ than O-. There were no significant differences in mRNA expression related to development. On the other hand, the level of ROS was increased while the mitochondrial activity of in vitro grown matured oocyte was less than in vivo matured oocytes. In conclusion, follicle culture with O+ group appears to be superior to the culture in O- group in ter...Continue Reading

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Citations

Jan 19, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Chunhua ZhanXuezhen Zhang
Jan 20, 2021·Journal of Clinical Laboratory Analysis·Xiyan WangXiaoyan Pan

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Methods Mentioned

BETA
reverse-transcription PCR
PCR
electrophoresis

Software Mentioned

Zen
Statistical Package for the Social Sciences
GraphPad Prism

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