Comparison of the pathogen species-specific immune response in udder derived cell types and their models

Veterinary Research
Juliane GüntherHans-Martin Seyfert

Abstract

The outcome of an udder infection (mastitis) largely depends on the species of the invading pathogen. Gram-negative pathogens, such as Escherichia coli often elicit acute clinical mastitis while Gram-positive pathogens, such as Staphylococcus aureus tend to cause milder subclinical inflammations. It is unclear which type of the immune competent cells residing in the udder governs the pathogen species-specific physiology of mastitis and which established cell lines might provide suitable models. We therefore profiled the pathogen species-specific immune response of different cell types derived from udder and blood. Primary cultures of bovine mammary epithelial cells (pbMEC), mammary derived fibroblasts (pbMFC), and bovine monocyte-derived macrophages (boMdM) were challenged with heat-killed E. coli, S. aureus and S. uberis mastitis pathogens and their immune response was scaled against the response of established models for MEC (bovine MAC-T) and macrophages (murine RAW 264.7). Only E. coli provoked a full scale immune reaction in pbMEC, fibroblasts and MAC-T cells, as indicated by induced cytokine and chemokine expression and NF-κB activation. Weak reactions were induced by S. aureus and none by S. uberis challenges. In contras...Continue Reading

References

Dec 1, 1994·Infection and Immunity·K R MatthewsS P Oliver
Jun 1, 1996·Journal of Dairy Science·R A AlmeidaS P Oliver
Mar 5, 2002·Nature Immunology·Michael Karin, Anning Lin
Sep 2, 2003·Cytokine & Growth Factor Reviews·Evemie SchutyserJo Van Damme
Sep 2, 2003·Journal of Leukocyte Biology·De YangJoost J Oppenheim
Sep 2, 2003·Nature Reviews. Immunology·Tomas Ganz
Oct 15, 2003·Veterinary Research·Christian BurvenichLuc Duchateau
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·T GoldammerH-M Seyfert
May 13, 2004·Clinical and Diagnostic Laboratory Immunology·Douglas D BannermanPascal Rainard
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Dec 12, 2007·Handbook of Experimental Pharmacology·Satoshi Uematsu, Shizuo Akira
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine WalshClare Bryant
Jul 1, 2009·Journal of Leukocyte Biology·RoseMarie StillieAndrew W Stadnyk
Feb 6, 2010·Nature Protocols·Igor UlitskyRon Shamir
Mar 23, 2010·Cell·Osamu Takeuchi, Shizuo Akira
May 11, 2010·Cellular and Molecular Life Sciences : CMLS·Daniela Barretto Barbosa TrivellaIgor Polikarpov
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Sep 6, 2011·European Heart Journal·Ulrich Förstermann, William C Sessa
Oct 1, 2011·Veterinary Immunology and Immunopathology·Ynte H SchukkenUNKNOWN members of the Pfizer mastitis research consortium
Oct 5, 2011·Journal of Mammary Gland Biology and Neoplasia·Ruth N ZadoksYnte H Schukken
Apr 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Umeharu OhtoToshiyuki Shimizu
Oct 23, 2012·Cancer Letters·Wen-Ming Chu

❮ Previous
Next ❯

Citations

Jun 5, 2016·Veterinary Microbiology·Kathleen E BuddOrla M Keane
Oct 27, 2018·Frontiers in Veterinary Science·Pascal Rainard, Gilles Foucras
May 10, 2017·BMC Genomics·Andreas LeimbachUlrich Dobrindt
May 29, 2018·Journal of Dairy Science·P RainardR Rupp
Sep 6, 2018·Seminars in Immunopathology·Juliane Günther, Hans-Martin Seyfert
Jul 3, 2021·International Journal of Molecular Sciences·Ting LiChangmin Hu

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
FCS
transfect
Transfection

Software Mentioned

Invivogen
Expander ( EXPression ANalyzer and DisplayER )
GraphPad
GraphPad Prism
MAC
EXPANDER

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.