Comparison of the performance of 2 commercial multiplex PCR platforms for detection of respiratory viruses in upper and lower tract respiratory specimens

Diagnostic Microbiology and Infectious Disease
Elisa CostaDavid Navarro

Abstract

The performance of the CLART® PneumoVir system with that of the Luminex xTAG RVP Fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n=183) from unique patients with influenza-like syndrome or lower tract respiratory infection. Nested PCR coupled to automated sequencing was used for resolution of discrepancies. Fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n=21) or fully discordant (n=35) results (Kappa coefficient, 0.62). The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, influenza A virus/H3N2, influenza B virus, human metapneumovirus, and parainfluenza virus, whereas the sensitivity of the latter was higher for most targeted viruses except, notably, for picornaviruses. This was irrespective of either the origin of the respiratory specimen or the age group to which the patients belonged.

References

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Citations

Sep 22, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·José Luis PiñanaDavid Navarro
Aug 11, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chikara OgimiMichael Boeckh
Apr 23, 2020·Journal of Medical Virology·María Luisa BlascoDavid Navarro
May 26, 2017·Transplant Infectious Disease : an Official Journal of the Transplantation Society·José Luis PiñanaCarlos Solano

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Methods Mentioned

BETA
nucleic acid extraction
PCR
DNA array assay
PCRs
DNA array

Software Mentioned

CLART® pneumoVir
xPONENT
CLART®

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