PMID: 7581362Aug 1, 1995Paper

Comparison of the positional cloning methods used to isolate the BRCA1 gene

Human Molecular Genetics
K HarshmanP Dayananth

Abstract

A critical step in positional cloning is the identification of candidate genes from a large, genetically defined region. Candidate gene isolation by hybrid selection, genomic sequencing, and direct cDNA library screening identified 45 candidate gene fragments (CGFs) from a 600 kb genomic region that contains the BRCA1 gene. These CGFs define a minimum of 15 genes, six of which are newly localized to the BRCA1 region. We present an analysis of the efficiency and the sequences generated for each of these methods. We also compare our CGF set to those reported for the BRCA1 region by three other groups, revealing a surprising lack of overlap among the sets.

Citations

Dec 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T HattierA Kamb
Sep 7, 2007·Molecular Biology of the Cell·Chun-Chun LiFang-Jen S Lee
Nov 5, 1999·The Nursing Spectrum·K A Calzone
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D J Prockop
Jul 8, 1998·Medicine·L C Brody, B B Biesecker

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