Comparison of the production of a human monoclonal antibody against HIV-1 by heterohybridoma cells and recombinant CHO cells: A flow cytometric study.

Cytotechnology
N BorthH Katinger

Abstract

The production of human monoclonal antibodies for therapeutic use is of increasing importance for treatment of viral infections such as AIDS. As human x mouse heterohybridomas rarely reach the growth rates and cell specific production rates of mouse hybridomas the transfection of standard cell lines, such as CHO or BHK, is a promising alternative. This has the additional advantage that the IgG subtype can be changed to suit the desired application. However, the use of a cell line that has not originally developed to produce antibodies, as lymphocytes and myeloma cells have, might have unrecognised drawbacks. This will be especially significant in the case of antibodies as each molecule consists of 4 chains linked by disulphide bonds which require specific intracellular factors to be properly folded and processed (Heavy chain binding protein, Protein Disulfide Isomerase a.o.). In this study we have therefore compared two cell lines: a human x mouse heterohybridoma producing IAM-2F5, a human IgG(3) antibody specific for HIV-1 with neutralising properties and a Chinese Hamster Ovary cell transfected with dihydrofolate reductase and with the heavy and light chain genes of IAM-2F5 modified to IgG(1). From each cell line three subclo...Continue Reading

References

Oct 1, 1988·Molecular and Cellular Biology·A J DornerR J Kaufman
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Nov 24, 1983·Nature·I G Haas, M Wabl
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Aug 1, 1994·Trends in Biochemical Sciences·R B FreedmanM F Tuite
Dec 20, 1989·Biotechnology and Bioengineering·E Suzuki, D F Ollis

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Citations

Oct 27, 2004·Expert Opinion on Biological Therapy·Silvia Carroll, Mohamed Al-Rubeai
Oct 24, 2003·Biotechnology and Bioengineering·Simone M SchatzFriedrich Scheiflinger

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