Comparison of the properties of the alcohol dehydrogenases from wild-type and mutant Chinese hamster somatic cells

Biochemical Genetics
B G Talbot, J P Thirion

Abstract

Alcohol dehydrogenases (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) from allyl alcohol-resistant and wild-type Chinese hamster cells were purified using gel filtration, ion-exchange, and affinity-column chromatography. Both enzymes exhibited the same isozyme band patterns on electrophoresis and isoelectric focusing. Physicochemical properties of the two enzymes such as pH and temperature optima, Km values, and temperature stability were found to be the same within the experimental errors. The genetic significance of these findings is discussed.

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D Schwartz
Jul 1, 1967·Archives of Biochemistry and Biophysics·R Megnet
Jun 15, 1973·Annals of the New York Academy of Sciences·A R WilliamsonH W Kreth
Jan 1, 1970·The Biochemical Journal·Z L AwdehB A Askonas
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

Citations

Oct 1, 1986·Biochemical Genetics·R BrzezinskiJ P Thirion

Related Concepts

Alcohol Oxidoreductases
Metazoa
Chromatography, Affinity
Molecular Sieve Chromatography
Ion-Exchange Chromatography Procedure
Cricetulus
SDS-PAGE
Cricetus
Hydrogen-Ion Concentration
Isoelectric Focusing

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