Comparison of the QMS Analyzer With HPLC-UV for the Quantification of Lamotrigine Concentrations in Human Plasma Samples

Therapeutic Drug Monitoring
Sara BaldelliEmilio Clementi

Abstract

Recently, a turbidimetric immunoassay method has been developed for use in the form of a QMS lamotrigine (LTG) commercial immunoassay. This study was designed to evaluate the performance of this immunoassay using a validated high-performance liquid chromatography-ultraviolet (HPLC-UV) method as the reference. The performance of QMS was initially tested using drug-free plasma spiked with different amounts of LTG and, subsequently, by analyzing 61 trough plasma samples from epileptic patients given the drug as part of their maintenance antiepileptic therapies. The correlation between LTG concentrations measured by QMS and HPLC was good, with a Pearson coefficient of 0.968 (P < 0.0001). The Bland-Altman approach showed that LTG concentrations measured with QMS exceeded HPLC on an average by 15.6% (limits of agreement, -18% to +63%), with a concentration-dependent performance (mean percent bias, 49.5 ± 8.2% and 0.6 ± 12.7% for concentrations less than 2 mg/L and greater than 14.9 mg/L, respectively). The QMS provided acceptable analytical performance across a wide concentration range for routine LTG measurements, being at least comparable with the other commercial immunoassays. It could be, therefore, considered as a viable alterna...Continue Reading

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Aug 26, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Sara BaldelliEmilio Clementi

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Citations

Nov 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karina Sommerfeld-KlattaFranciszek K Główka
Mar 7, 2021·Critical Reviews in Analytical Chemistry·Sajeeda SRuchi Verma
Jul 9, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kwabena F M OpuniCharles A Okai
Mar 16, 2021·Archives of Pathology & Laboratory Medicine·Matthew D KrasowskiStacy E F Melanson

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