Comparison of the rate of sequence variation in the hypervariable region of E2/NS1 region of hepatitis C virus in normal and hypogammaglobulinemic patients

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
J C BoothH C Thomas

Abstract

The hypervariable region (HVR) of the E2/NS1 region of hepatitis C virus (HCV) varies greatly between viral isolates with high rates of genomic change reported during the course of chronic infection. The HVR is thought to encode a structurally unconstrained envelope protein containing several linear B cell epitopes recognized by neutralizing antibody. It has been postulated that amino acid changes in the HVR could result from humoral immune pressure leading to the selection of escape mutants. The aim of this study was to compare the rates of nucleotide and amino acid variation in the HVR of control patients to patients with common variable immunodeficiency (CVID) where the effect of the humoral immune system is reduced. Five controls and four patients with CVID were studied. Serum samples were taken over periods of between 1 and 6 years. HCV was detected by polymerase chain reaction (PCR) with primers derived from conserved flanking regions of the HVR. PCR products were cloned into a plasmid vector and recombinant clones identified by restriction enzyme digestion. Purified DNA from at least three individual clones from each time point was sequenced by the dideoxynucleotide chain-termination method. Consensus sequences were extr...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A J WeinerK A Crawford
Sep 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S OkadaS Mishiro
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T F WolfsJ Goudsmit
Feb 28, 1991·Biochemical and Biophysical Research Communications·M HijikataK Shimotohno
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N OgataR H Purcell
Jun 1, 1993·Journal of Medical Virology·R R LesniewskiI K Mushahwar

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Citations

Aug 13, 1998·Journal of Medical Virology·L G BirkenmeyerI K Mushahwar
Mar 14, 2000·Reviews in Medical Virology·M Flint, J A McKeating
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K E ShermanD Thee
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M ChristieW M Rosenberg
May 8, 2003·Journal of Biosciences·Nicole Pavio, Michael M C Lai
Jan 6, 2000·Journal of Hepatology·F Zoulim
Sep 28, 1999·Trends in Microbiology·X FornsJ Bukh
Apr 15, 2011·Expert Reviews in Molecular Medicine·Viet Loan Dao ThiFrançois-Loïc Cosset
Sep 11, 2002·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S Einav, M J Koziel
Oct 28, 1998·Vox Sanguinis·H RollagJ L Svennevig
Aug 19, 2007·Journal of Virology·Thomas KuntzenTodd M Allen
Apr 29, 2011·Journal of Virology·Sumathi RamachandranYury E Khudyakov
Mar 16, 2005·Journal of Virology·Belinda L HerringEric L Delwart
May 21, 2011·BMC Evolutionary Biology·Rebecca R GrayOliver G Pybus
Dec 14, 2011·Viruses·Caterina Di LorenzoArvind H Patel
Jun 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·C LogvinoffJ A McKeating
Feb 25, 2014·Archives of Virology·Regina Maria Santos de AmorimRegina Maria Bringel Martins
Jun 29, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Henry PollackWilliam Borkowsky
Sep 7, 2004·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Anna FeliuXavier Forns
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M U MondelliE Silini
Jul 16, 2008·Clinics in Liver Disease·Zania StamatakiJane A McKeating
Aug 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Loredana FrascaEnza Piccolella
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Antonio Bertoletti, Carlo Ferrari
Mar 12, 2003·Journal of Medical Virology·Emanuele Durante MangoniPeter Karayiannis

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