Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high virulent and low virulent strains of Edwardsiella tarda

Fish & Shellfish Immunology
Keiko IshibeTatsuya Oda

Abstract

In vivo infection studies in Japanese flounder (Paralichthys olivaceus) demonstrated that the number of viable cells of the virulent strain (NUF251) of Edwardsiella tarda increased gradually in kidney and hepato-pancreas after intraperitoneal injection, but the low virulent strain (NUF194) did not. To gain insight into the virulence factors of E. tarda, in vitro responses of Japanese flounder (P. olivaceus) peritoneal macrophages to these strains were compared in terms of phagocytosis, bactericidal activity, and reactive oxygen species (ROS) generation as measured by chemiluminescence (CL) responses. Microscopic observation revealed that these two strains of E. tarda were phagocytosed by the peritoneal macrophages, and there was no significant difference in the mean numbers of ingested bacteria per macrophage between these strains. A gradual increase in the number of viable cells of the highly virulent strain within macrophages was observed during 9h post-phagocytosis, whereas no significant replication of the low virulent strain within macrophages was detected. These results suggest that the virulent strain of E. tarda has an ability to survive and replicate within macrophages, while the low virulent strain has no such ability...Continue Reading

References

Jan 1, 1989·Archives of Microbiology·M Sanchez-MorenoM Monteoliva
Oct 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J M Janda, S L Abbott
May 1, 1997·Molecular Microbiology·I HironoT Aoki
Dec 27, 2005·Fish & Shellfish Immunology·Kazushi KadomuraTatsuya Oda

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Citations

Apr 17, 2013·Fish & Shellfish Immunology·Khriezhato NakhroDibyendu Kamilya
Mar 16, 2013·Veterinary Immunology and Immunopathology·Masatoshi YamasakiAtsushi Yamamoto
Oct 6, 2012·Veterinary Research·Seong Bin ParkTae Sung Jung
Aug 21, 2013·Developmental and Comparative Immunology·Leon GrayferMiodrag Belosevic
Feb 27, 2014·Veterinary Research·Yong-Hua HuLi Sun
Apr 29, 2015·Developmental and Comparative Immunology·Beibei ZhaoIkuo Hirono
Jul 30, 2016·Frontiers in Cellular and Infection Microbiology·Ze-Jun Zhou, Li Sun
Sep 30, 2019·Microbiological Research·Priyanka AggarwalKa Yin Leung

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