PMID: 16536003Mar 16, 2006Paper

Comparison of the results of M. tuberculosis cultures on liquid media in MB/BacT system and solid Löwenstein Jensen media in selected patients

Pneumologia : revista Societății Române de Pneumologie
Daniela Homorodean

Abstract

The study was performed to evaluate the performances of the MB/BacT System in comparison to solid media Löwenstein Jensen for the recovery and the time for the detection of mycobacteria from specimens of selected patients from a dust and chemical polluted area, where the incidence of tuberculosis is higher than the mean county incidence- as control- and silicotuberculosis patients. We processed 193 selected specimens (156 as control and 37 from silicotuberculosis patients). All specimens have been processed for microscopic examination, culture on Löwenstein Jensen media and in Middlebrook7H9 liquid media in automatic system MB/BacT Sputum, bronchial aspirate and tongue swab had been previously decontaminated, but aseptically obtained specimens not, before inoculation onto the culture media. The culture positivity mean time for control was 14,56 days in MB/BacT system and 28.66 days on Löwenstein Jensen, and 17.7 days, and 77 days respectively for silicotuberculosis patients specimens, without significant difference between the two groups (chi2 = 0,33; p = 0,565; IC95). Simultaneously culture of selected specimens by using two different culture media for Mycobacterium strains isolation can contribute (it the improvement of the r...Continue Reading

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