Comparison of the salt-dependent self-association of brain and erythroid spectrin

Biochemistry
G E BeggG B Ralston

Abstract

The self-association of ovine brain spectrin in 0.1-1.5 M NaCl (pH 7.5) was studied using sedimentation velocity and sedimentation equilibrium techniques. Brain spectrin is tetrameric at sedimentation equilibrium at a 0.13 M ionic strength at 18-37 degrees C and at ionic strengths of up to 0.33 M at 20 degrees C. At ionic strengths greater than 0.33 M at 20 degrees C, the brain spectrin tetramer is destabilized, resulting in both dissociation to dimers and indefinite association to higher oligomers, in a manner similar to that seen with erythroid spectrin. The equilibrium constants describing all steps in the association involving the addition of dimers are around 15-fold higher for brain spectrin than for erythroid spectrin, at ionic strengths of > or = 0.43 M. We propose that the stronger association of brain spectrin compared to that of erythroid spectrin is due to a relative inability of brain spectrin to form closed dimers. Sedimentation velocity analysis confirms that brain spectrin readily forms open dimers and that the association of open dimers is not kinetically trapped even at 2 degrees C. Our results suggest that the destabilization of spectrin tetramers in high-ionic strength conditions is due to increased independ...Continue Reading

References

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Citations

Oct 24, 2006·Journal of Biomolecular Structure & Dynamics·Malyasri BhattacharyaAbhijit Chakrabarti
Jun 1, 2002·European Journal of Haematology·Bing-Hao LuoL W-M Fung
Jul 18, 2015·European Biophysics Journal : EBJ·Madhurima MitraAbhijit Chakrabarti
Oct 2, 2007·Protein Science : a Publication of the Protein Society·Fei LongLeslie W-M Fung
Aug 26, 2011·Cellular & Molecular Biology Letters·Akin Sevinc, Leslie W-M Fung
Sep 17, 2009·Cellular & Molecular Biology Letters·Jianxia KangLeslie W-M Fung
Oct 10, 2006·Bioscience Reports·Abhijit ChakrabartiAmitabha Chattopadhyay
Jan 11, 2014·Journal of Biomolecular Structure & Dynamics·Malay PatraChaitali Mukhopadhyay
Jun 13, 2015·PLoS Computational Biology·Jeffrey W BrownC James McKnight
Apr 4, 2003·The Journal of Biological Chemistry·Sunghyouk ParkLeslie W-M Fung

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