Comparison of the Seeplex HPV4A ACE and the Cervista HPV assays for the detection of HPV in hybrid capture 2 positive media.

Journal of Gynecologic Oncology
Kyung-Jin MinAe-Ree Kim

Abstract

To validate the efficacy of Seeplex HPV4A ACE for the detection of high-risk (HR) human papillomavirus (HPV) and HPV 16 and/or HPV 18 genotypes as compared to the PCR method and the Cervista HPV assays in cervical swab samples. Besides liquid-based cytology, additional 97 cervical swab samples were collected for HPV genotyping by HPV4A ACE, Cervista HPV assays, and PCR method. To check the statistical differences, we also conducted the paired proportion test, Cohen's κ statistic, and a receiver operating characteristic curve. Seeplex HPV4A ACE and the Cervista HPV HR showed substantial agreement with PCR for detection of HR HPVs (88.3%, κ=0.767 and 81.7%, κ=0.636, respectively). Seeplex HPV4A ACE also showed substantial agreement with the Cervista HPV 16/18 test (89.5%, κ=0.628). Additionally, the sensitivity and specificity of Seeplex HPV4A ACE and Cervista HPV HR were 91.4% vs. 84.5% and 73.4%, vs. 72.7%, respectively, when those higher than low-grade squamous intraepithelial lesions were regarded as abnormalities. HPV genotyping for HPV 16/18 detected cervical intraepithelial neoplasias (CINs) better than HR HPV tests (66.7% vs. 24.6% by HPV4A ACE, 52.6% vs. 25.9% by Cervista HPV assays in CIN II or more, relatively). Seeple...Continue Reading

References

Mar 16, 2000·Journal of the National Cancer Institute·R HerreroM Schiffman
Jan 31, 2003·British Journal of Cancer·G M CliffordS Franceschi
Jun 17, 2003·Journal of the National Cancer Institute. Monographs·Mark Schiffman, Susanne Krüger Kjaer
Apr 16, 2005·The Lancet Oncology·Vincent CoglianoUNKNOWN WHO International Agency for Research on Cancer
Sep 11, 2007·Lancet·Mark SchiffmanSholom Wacholder
Nov 27, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·L Z MoC Mougin
Feb 12, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Cristina BaleriolaWilliam Rawlinson
Aug 11, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephen P DayMarilyn Olson
Aug 20, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Fatima Galan-Sanchez, Manuel A Rodriguez-Iglesias

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Methods Mentioned

BETA
genotyping
PCR
biopsy
electrophoresis
Chip

Software Mentioned

Seegene viewer
Seeplex
dbSTAT

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