Comparison of the sequences of the Aspergillus nidulans hxB and Drosophila melanogaster ma-l genes with nifS from Azotobacter vinelandii suggests a mechanism for the insertion of the terminal sulphur atom in the molybdopterin cofactor

Molecular Microbiology
L AmraniV Finnerty


The molybdopterin cofactor (MoCF) is required for the activity of a variety of oxidoreductases. The xanthine oxidase class of molybdoenzymes requires the MoCF to have a terminal, cyanolysable sulphur ligand. In the sulphite oxidase/nitrate reductase class, an oxygen is present in the same position. Mutations in both the ma-l gene of Drosophila melanogaster and the hxB gene of Aspergillus nidulans result in loss of activities of all molybdoenzymes that necessitate a cyanolysable sulphur in the active centre. The ma-l and hxB genes encode highly similar proteins containing domains common to pyridoxal phosphate-dependent cysteine transulphurases, including the cofactor binding site and a conserved cysteine, which is the putative sulphur donor. Key similarities were found with NifS, the enzyme involved in the generation of the iron-sulphur centres in nitrogenase. These similarities suggest an analogous mechanism for the generation of the terminal molybdenum-bound sulphur ligand. We have identified putative homologues of these genes in a variety of organisms, including humans. The human homologue is located in chromosome 18.q12.


Mar 21, 1977·Biochemical and Biophysical Research Communications·F H Gaertner, K W Cole
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 11, 1992·Nucleic Acids Research·P KamdarV Finnerty
Jun 11, 1991·Nucleic Acids Research·H BrodyW E Timberlake
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H Brody, J Carbon
Jul 1, 1967·The Biochemical Journal·J A PATEMAND J COVE
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·V G FinnertyA Chovnick
Aug 1, 1970·Genetical Research·M J Hartley
May 8, 1965·Nature·A J DarlingtonJ A PATEMAN
Dec 1, 1983·Gene·J TilburnR W Davies
Nov 1, 1993·Journal of Bacteriology·D R DeanL Zheng
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ZhengD R Dean
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·R HuberM J Romão
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 1, 1997·Biochemical Society Transactions·K P KamdarV Finnerty
Feb 17, 1999·FEMS Microbiology Reviews·P J Kiley, H Beinert
Nov 7, 1996·Chemical Reviews·Russ Hille


Apr 10, 2007·Plant Molecular Biology·Ana Paula Guedes FrazzonBrenda S J Winkel
Sep 5, 2002·Free Radical Biology & Medicine·Roger Harrison
Mar 26, 2003·Insect Biochemistry and Molecular Biology·N KômotoH Fujii
Aug 8, 2002·The Plant Journal : for Cell and Molecular Biology·M SagiRobert Fluhr
May 4, 2006·Annual Review of Plant Biology·Günter Schwarz, R R Mendel
Feb 22, 2008·Biology Direct·Daniel H Haft, William T Self
Dec 4, 2012·International Journal of Molecular Sciences·Kimiyoshi IchidaT Nishino
Apr 17, 2001·Biochemical and Biophysical Research Communications·K IchidaT Nishino
Dec 15, 2010·Biochemistry Research International·Meina Neumann, Silke Leimkühler
Mar 4, 2008·Molecular Microbiology·Stanislav G KozminRoel M Schaaper
Mar 23, 2011·Fungal Genetics and Biology : FG & B·Christos GournasC Scazzocchio
Jun 28, 2014·Fungal Genetics and Biology : FG & B·Katerina GalanopoulouSotiris Amillis
Mar 2, 2017·Redox Report : Communications in Free Radical Research·Natalia V PerkhulynVolodymyr I Lushchak
Mar 21, 2007·Molecular Microbiology·Antonietta CultroneRafael Fernández-Martín
Apr 7, 2006·The Journal of Biological Chemistry·Meina NeumannSilke Leimkühler
Feb 18, 2004·Journal of Environmental Quality·Ami L WangelineElizabeth A H Pilon-Smits

Related Concepts

Molybdenum cofactor
NifS protein, Bacillus subtilis
Aspergillus nidulans
Bacterial Proteins
Drosophila melanogaster
Genes, Bacterial

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