Comparison of the serum metabolic signatures based on 1 H NMR between patients and a rat model of deep vein thrombosis

Scientific Reports
Jie CaoJun-Hong Sun

Abstract

Deep vein thrombosis (DVT) and pulmonary embolism (PE) have high morbidity, reduce quality of life, and can cause death. Biomarkers or genetic risk factors have not been identified in patients with DVT. In present study, serum of 61 patients suffering from DVT and a rat DVT model (n = 10) were assayed by a proton nuclear magnetic resonance (1H NMR) metabolomics technique combing with multivariate statistical analysis to identify the metabolites. The MetPA platform was used to identify differences in the metabolic pathways between the rat model and patients. The metabolomics results discovered that 11 different metabolites in rats and 20 different metabolites in DVT patients. Seven metabolites both altered in the rats and patients. Moreover, we observed changes in the metabolic pathways, including carbohydrate metabolism, lipid metabolism, and amino acid metabolism that were induced immediately by the thrombosis. Pathway of aminoacyl-tRNA biosynthesis perturbed only in the patients which was associated with the genetic risk factor of DVT. The study demonstrated that serum 1H NMR metabolomics can be used to diagnose DVT in the clinic. The altered pathways related to thrombosis and genetics will provide a foundation and new strate...Continue Reading

References

Apr 21, 1999·Lancet·F R Rosendaal
Aug 31, 2000·Annual Review of Biochemistry·M Ibba, D Soll
Apr 12, 2003·American Journal of Human Genetics·Anthony AntonellisEric D Green
Dec 13, 2006·Journal of Thrombosis and Haemostasis : JTH·M Di NisioP M M Bossuyt
Mar 21, 2007·Journal of Thrombosis and Haemostasis : JTH·I A NaessJ Hammerstrøm
Sep 5, 2008·Annual Review of Genomics and Human Genetics·Anthony Antonellis, Eric D Green
Oct 25, 2008·Nature·Jeremy K Nicholson, John C Lindon
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·F R Rosendaal, P H Reitsma
Mar 20, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Benjamin O PattersonPeter J E Holt
Jul 14, 2010·Journal of the American College of Cardiology·Samuel Z Goldhaber
Aug 10, 2010·Wiener medizinische Wochenschrift·Martina Kronberger
Feb 22, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Pieter H ReitsmaSaskia Middeldorp
Apr 13, 2012·Lancet·Samuel Z Goldhaber, Henri Bounameaux
Feb 22, 2013·EMBO Molecular Medicine·Peng Yao, Paul L Fox
Dec 26, 2013·Journal of Proteome Research·Renata BujakCoral Barbas
Jul 6, 2014·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Manal F GhozlanSoha Abuelela
Apr 22, 2015·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Apr 29, 2015·Molecular BioSystems·Mohamad Hafizi Abu BakarHarisun Yaakob
Dec 18, 2015·Journal of the Science of Food and Agriculture·Jian LiuJia-Shun Gong
Sep 8, 2016·Current Protocols in Bioinformatics·Jianguo Xia, David S Wishart
Aug 30, 2017·International Journal of Molecular Medicine·Qian-Qian JinYing-Yuan Wang
Jan 25, 2018·Journal of Inherited Metabolic Disease·Clara D M van KarnebeekKym M Boycott

❮ Previous
Next ❯

Citations

Oct 17, 2019·Journal of Proteome Research·Sarah OnidaAlun H Davies

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
NMR

Software Mentioned

MestReNova
Metscape
DA
MetPA
MetaboAnalyst
SIMCA
PLS
OPLS
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.