Comparison of the structural requirements for bacteriochlorophyll binding in the core light-harvesting complexes of Rhodospirillum rubrum and Rhodospirillum sphaeroides using reconstitution methodology with bacteriochlorophyll analogs

Biochemistry
C M DavisP A Loach

Abstract

Bacteriochlorophyll (BChl) structural requirements for formation of the core light-harvesting complex (LH1) and its structural subunit complex were examined by reconstitution with BChl analogs and the alpha- and beta-polypeptides of Rhodospirillum rubrum and Rhodobacter sphaeroides. Comparable results were obtained with most of the BChl analogs and the polypeptides of each bacterium, indicating the conservation of BChl binding sites. These systems showed the following common requirements for formation of the subunit complex and LH1: (1) Mg or a metal of similar size and coordination chemistry (e.g., Zn, Cd, Ni), (2) a bacteriochlorin oxidation state of the macrocyclic ring, (3) a 13(2)-carbomethoxy group, and (4) an intact ring V. Some structural features were not as critically important. For example, the subunit complex and LH1 could be formed with both sets of polypeptides and BChl b, as well as with analogs containing either short (ethanol) or long (phytol) esterifying alcohols. Two derivatives were identified that behave differently with the two sets of polypeptides. The 3-acetyl group is required to form LH1 in both bacteria, although a subunit-type complex was readily formed with [3-vinyl]BChl a and the polypeptides of Rs...Continue Reading

References

Aug 14, 1992·Journal of Photochemistry and Photobiology. B, Biology·R A Brunisholz, H Zuber
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·J Deisenhofer, H Michel
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·W J Coleman, D C Youvan

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Citations

Jun 8, 2001·European Journal of Biochemistry·H K RauW Haehnel
Jul 26, 2013·The Journal of Chemical Physics·Daisuke KosumiHideki Hashimoto
Jul 25, 2006·Biochimica Et Biophysica Acta·Thomas VolkmerDirk Schneider
Jul 30, 2021·Biochemistry·Kazutoshi TaniZheng-Yu Wang-Otomo

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