Comparison of the transcellular transport of FDG and D-glucose by the kidney epithelial cell line, LLC-PK1

Nuclear Medicine Communications
Masato KobayashiKeiichi Kawai

Abstract

Almost all D-glucose (GLU) filtered through the glomerulus is reabsorbed by the renal proximal tubules, whereas a high portion of 2-[18F]fluoro-2-deoxy-D-glucose [(18F)FDG] is excreted through the urine. However, [18F]FDG is not entirely excreted in the urine suggesting that it may be partially reabsorbed by the proximal tubules. The purpose of this study was to compare the time course of transcellular transport of administered [14C] labeled FDG ([14C]FDG) with that of [14C] labeled GLU ([14C]GLU) using the kidney epithelial cell line, LLC-PK1. Transcellular transport of [14C]FDG and [14C]GLU by LLC-PK1 cells was measured in Na+-containing or Na+-free Dulbecco's phosphate-buffered saline [PBS(+) and PBS(-), respectively] in the presence or absence of phlorizin, phloretin, probenecid, or tetraethylammonium bromide inhibitors that predominantly inhibit sodium-dependent glucose transporters (SGLTs), sodium-independent glucose transporters, organic anion transporters, and organic cation transporters, respectively. When assayed in PBS(+), less [14C]FDG than [14C]GLU was reabsorbed by the proximal tubular cells over the entire incubation time. Reabsorption of [14C]FDG was mediated mainly by SGLT at early time points in the incubation...Continue Reading

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Citations

Apr 8, 2011·Nihon Hoshasen Gijutsu Gakkai zasshi·Keiichi Kawai
Nov 30, 2010·Seminars in Nuclear Medicine·Zsolt SzaboHamid Rabb
Feb 21, 2021·Nuclear Medicine and Biology·Masato KobayashiKeiichi Kawai

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